课题基金 / 基金详情

项目摘要

项目成果

Karin D Rodland的其他基金

相似基金

相关文献

中文摘要
翻译
PNNL临床蛋白质组学表征中心的总体目标
英文摘要
The Overall Objective ofthe PNNL Clinical Proteome Characterization Center (PCPCC) is to facilitate cancer biomarker development by linking the cancer genotype to the cancer phenotype using detailed comprehensive and quantitative characterization of cancer proteomes to complement the extensive genome-level characterization provided by The Cancer Genome Atlas (TCGA). The PCPCC will contribute to the success ofthe planned network of Protein Characterization Centers (PPCs) by utilizing robust and quantitative proteomics technologies and workflows, including simultaneous application of state-of-the-art validated platforms and advanced developmental platforms, for systematic discovery and verification of protein biomarkers that can be qualified in clinical studies, using the cancer specimens and associated data provided by the CPTC. The Discovery Unit will make measurements providing a comprehensive and quantitative characterization ofthe cancer proteomes that provides Information including protein abundances, splicing variants, mutations, and posttranslational modifications to complement the genomic characterization for CPTC-supplied biospecimens. The extensive database of genomic information on these samples will be integrated with the quantitative proteomic measurements made by the PCPCC, other available proteomics Information (e.g., from other PCC's), and a systems-level analysis of tumor-speclfic pathways to produce a prioritized list of highly credentialed candidates based on a weighted integration of multiple sources of Information, including clinical oncology and cancer biology. The Verification Unit will systemically develop and apply multiplexed verification assays directed at specific protein targets as identified and selected by the Biomarker Candidate Selection Subcommittee. The PCPCC will develop sensitive, selective, quantitative assays for a minimum of 100 protein targets per year and apply ultra-sensitive assays to biomari<er verification with a throughput of at least 500 plasma (or serum) samples per year, for a total of at least 2500 samples. Additionally, as in the Discovery efforts, measurements with the best available validated platform will be augmented by measurements with a developmental high performance platform for the same samples (for an overall total of at least 1000 samples per year, and >5000 total) to provide quantitative measurements for low-abundance otherwise undetectable candidates. As part of a consortium of PCC's, the PCPCC will also work to advance the efforts of others based upon e.g. the cancer tumor proteomics data generated, as well as subsequent biomarker clinical qualification and validation efforts. RELEVANCE (See instructions): Despite recent declines in the cancer death rate, cancer remains a significant source of mortality: 25% of all deaths In the US are attributed to cancer. There Is a real clinical need for eariier diagnosis, more accurate prognosis, and more effective therapeutic targeting to improve the outcome for patients with cancer. The PCPCC is dedicated to using the best state-of-the-art and advanced developmental MS platforms and methods, systems biology approaches, and clinical collaborations, to advance the discovery and verification of cancer biomarkers.
期刊论文(7)
专著(0)
科研奖励(0)
会议论文
DOI: 10.1016/j.tibs.2014.10.010
发表时间: 2015-01
期刊: Trends in biochemical sciences
影响因子: 13.8
作者: [Payne SH]
通讯作者: Payne SH
DOI: 10.3390/cells10112823
发表时间: 2021-10-21
期刊: Cells
影响因子: 6
作者: [Huang MF, Pang LK, Chen YH, Zhao R, Lee DF]
通讯作者: Lee DF
DOI: 10.1517/17530059.2012.718329
发表时间: 2013-01
期刊: Expert opinion on medical diagnostics
影响因子: --
作者: [McDermott JE, Wang J, Mitchell H, Webb-Robertson BJ, Hafen R, Ramey J, Rodland KD]
通讯作者: Rodland KD
DOI: 10.1186/1471-2164-14-s8-s1
发表时间: 2013
期刊: BMC genomics
影响因子: 4.4
作者: [Zhang B, Huang Y, McDermott JE, Posey RH, Xu H, Zhao Z]
通讯作者: Zhao Z
Support for US HUPO Meeting "Future of Proteomics"
Deep Proteomics of Normal Human Ovarian Surface Epithelium and Fallopian Tube Epi
Center for Application of Advanced Clinical Proteomic Technologies for Cancer
Center for Application of Advanced Clinical Proteomic Technologies for Cancer
海外基金