Core C - Arrigoni

项目摘要/摘要 电生理学核心将为电生理学提供集中服务 研究，包括体外脑片的制备，膜片钳记录， 免疫组织化学方法，并进行相关数据分析。项目1(萨珀)、项目2 (Scammell)和Project 4(Chamberlin)将使用这一核心设施。我们将使用补丁- 钳位记录和通道视紫红质2(ChR2)辅助电路映射(CRACM)以 对于项目1，确定CGRP表达之间的功能性突触连接 臂旁外侧核神经元和基底核神经元 前脑(BF)、下丘脑外侧核(LH)、乳突上核(SUM)和中央 杏仁核(CEA)。对于项目2，我们将确定功能性突触连接 背外侧区胆碱能、谷氨酸能和GABA能神经元之间的相互作用 桥脑脚被盖核(LDT/PPT)与丘脑中线BF神经元 和总和，以及项目4在大脑皮质谷氨酸能前运动神经元之间。 舌下带和舌下运动神经元。在这些实验中，我们将 定义与这些输入有关的神经递质和突触后受体。在……里面 此外，核心将继续验证和优化刺激 光遗传学和药物遗传学工具的范例(ChR2和ARCHT兴奋性 和抑制性DREADD)由这些项目使用。这些项目将受益于 核心的专业知识和共享资源。

Project Summary/Abstract The Electrophysiology Core will provide centralized services for electrophysiological studies, including preparation of in vitro brain slices, patch-clamp recordings, immunohistochemistry, and the associated data analysis. Project 1 (Saper), Project 2 (Scammell) and Project 4 (Chamberlin) will use this Core facility. We will use patch- clamp recordings and channelrhodopsin2 (ChR2)-assisted circuit mapping (CRACM) to determine, for Project 1, functional synaptic connectivity between CGRP expressing neurons of the external lateral parabrachial nucleus (PBel) and neurons of the basal forebrain (BF), lateral hypothalamus (LH), supramammillary nucleus (SUM) and central amygdala (CeA). For Project 2 we will determine functional synaptic connectivity between cholinergic, glutamatergic and GABAergic neurons of the laterodorsal and pedunculopontine tegmental nuclei (LDT/PPT) and neurons of the BF, midline thalamus and SUM, and for Project 4 between glutamatergic premotorneurons of the parahypoglossal zone and hypoglossal motoneurons. In these experiments we will define the neurotransmitters and postsynaptic receptors involved in these inputs. In addition, the Core will be continuing the validation and optimization of the stimulation paradigms for the opto-genetic and pharmaco-genetic tools (ChR2 and ArchT excitatory and inhibitory DREADDs) used by these Projects. The Projects will benefit from the expertise and shared resources of the Core.