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Core C

核心C

基本信息

批准号：
10491084
负责人：
Elda Arrigoni
金额：
$ 17.38万
依托单位：
BETH ISRAEL DEACONESS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-01 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10491084
关键词：
Bathing Brain Calcium Cells Characteristics Clozapine Code Core Facility Cre driver Data Electrophysiology (science)Ensure Graph Histological Techniques Histology Image Imaging Techniques In Vitro Ivermectin Laboratories Light Measures Monitor Mus Nerve Neurons Neurotransmitters Oxides Physiologic pulse Population Population Study Property Protocols documentation Publications Publishing Reporter Research Design Research Personnel Resource Sharing Resources Services Sleep Apnea Syndromes Slice Statistical Data Interpretation Synapses Techniques Update Whole-Cell Recordings Work adeno-associated viral vector antagonist calcium indicator designer receptors exclusively activated by designer drugs efficacy testing experimental study extracellular imaging study in vivo neurochemistry novel optogenetics patch clamp postsynaptic postsynaptic neurons receptor response tool

项目摘要

Project Summary/Abstract- Core C The in vitro Electrophysiology Core (Core C) will provide services for in vitro electrophysiological studies using novel tools such as optogenetics, designer receptors exclusively activated by designer drugs (DREADDs) and in vitro GCaMP6 calcium imaging. We will prepare brain slices, conduct patch-clamp recordings, optimize the protocols for the optogenetic and chemogenetic experiments, perform Ca2+ imaging studies in in vitro slices, and conduct channelrhodopsin2 (ChR2)-assisted circuit mapping (CRACM). The Core will also perform histology of the recorded slices, and analyze the associated data. Project 1 (Saper), Project 2 (Scammell), Project 3 (Fuller) and Project 4 (Vanderhost and Dymecki) will use this Core facility. These projects are not equipped to conduct in vitro electrophysiology recordings and there are no institutional resources that can provide this service. The Projects will benefit from the expertise and shared resources of the Core.

项目概要/摘要-核心C 体外电生理核心（核心C）将为体外电生理学研究使用新的工具，如光遗传学，设计受体专门由设计药物（DREADD）和体外GCaMP 6钙激活显像我们将准备大脑切片，进行膜片钳记录，优化光遗传学和化学遗传学实验的方案，进行Ca 2+成像研究在体外切片，并进行通道视紫红质2（ChR 2）辅助电路映射（CRACM）。Core还将对记录的切片进行组织学检查，分析相关数据。项目1（Saper）、项目2（Scammell）、项目3（Fuller）项目4（Vanderhost和Dymecki）将使用该核心设施。这些项目未配备进行体外电生理学记录的设备，可以提供这种服务的机构资源。这些项目将受益于核心的专业知识和共享资源。