BD FACSAria Fusion Cell Sorter

BD FACSAria 融合细胞分选仪

• 批准号: 9076119
• 负责人: Michael R. Lee
• 金额: $ 57.28万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-03-15 至 2017-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9076119
• 关键词: Area; Cell Separation; Cell fusion; Cells; Clinical; Collecting Cell; Communities; Complement; Developmental Biology; Equipment; Evaluation; Faculty; Flow Cytometry; Funding; Grant; Hour; Human; Immunology; Minor; Modeling; Operating System; Research; Research Personnel; Research Project Grants; Resource Sharing; Sampling; Sorting - Cell Movement; Speed; United States National Institutes of Health; Update; base; cancer stem cell; career; design; flexibility; instrument; meetings; next generation; stem cell biology; tool

 DESCRIPTION (provided by applicant): We are requesting funds to purchase a next generation, user-operated Fluorescent Activated Cell Sorter that will both increase the cell sorting capacity at UCSF and update the capability of our current cell sorters in the Parnassus Flow Cytometry Core (PFCC). We have selected the Becton Dickenson FACSAria Fusion as the instrument that best fulfills the needs of our NIH-funded faculty. This cell sorter exceeds the PFCC's most sophisticated core-operated systems while maintaining ease of use; has biosafety and aseptic features that meet an increasing demand for sorting primary human cells and will enable UCSF researchers to directly sort and collect cells at high speeds with flexible configurations. Purchase of a FACSAria Fusion for the PFCC will provide maximal benefit to the entire, NIH-funded research enterprise at UCSF. Currently, each user-operated cell sorter in our shared resource facility averages 40 hours of use per week, severely limiting availability for new projects and for sorting of human clinical samples that are often obtained on short notice. High-speed cell sorting has become an essential tool in many fields, particularly immunology, developmental biology, cancer, and stem cell biology; and limited access to multiparameter high-speed cell sorting at UCSF, particularly on the Parnassus campus, has become a bottleneck for research progress. This limitation in cell sorting capacity impacts many investigators in numerous departments and research areas, new and early- career investigators are particularly hard-hit. In this application, we have identified 22 Investigators (19 major and 3 minor users, with 20 users funded by NIH grants) whose 27 research projects require increased access to cell sorting, as well as the specific capabilities of the FACSAria Fusion that are not provided by older Cell Sorters in our facility. Based on our evaluation of the needs of these investigators and availability of sorters and their configurations at the Parnassus campus of UCSF, we have decided to request a new BD FACSAria Fusion sorter with the configuration detailed in the equipment section. The choices on the make, model, and configuration, and operational plans are designed to complement existing sorters and maximally benefit investigators defined in this application as well as the entire research community at UCSF.

 描述（由申请人提供）：我们正在申请资金购买下一代，用户操作的荧光激活细胞分选仪，这将增加UCSF的细胞分选能力，并更新我们目前的细胞分选仪在Parnassus流式细胞术核心（PFCC）的能力。我们选择Becton Dickenson FACSAria Fusion作为最能满足NIH资助教师需求的仪器。这个细胞分选仪超过了 PFCC最先进的核心操作系统，同时保持易用性;具有生物安全和无菌功能，可满足对分选原代人类细胞日益增长的需求，并将使UCSF研究人员能够以灵活的配置高速直接分选和收集细胞。为PFCC购买FACSAria Fusion将为UCSF的整个NIH资助的研究企业提供最大的利益。目前，在我们的共享资源设施中，每个用户操作的细胞分选机平均每周使用40小时，严重限制了新项目的可用性，以及通常在短时间内获得的人类临床样本的分选。高速细胞分选已成为许多领域的重要工具，特别是免疫学，发育生物学，癌症和干细胞生物学;在UCSF，特别是在Parnassus校园，获得多参数高速细胞分选的机会有限，已成为研究进展的瓶颈。细胞分选能力的这种限制影响了许多部门和研究领域的许多研究者，新的和早期职业研究者受到的打击尤其严重。在本申请中，我们确定了22名研究人员（19名主要研究人员和3名 少数用户，其中20个用户由NIH赠款），其27个研究项目需要增加细胞分选的访问权限，以及FACSAria Fusion的特定功能，这些功能是我们设施中较旧的细胞分选仪所不提供的。根据我们对这些研究人员的需求以及UCSF Parnassus校区分拣机及其配置的可用性进行的评估，我们决定申请一台新的BD FACSAria Fusion分拣机，其配置详见设备部分。对制造，模型和配置以及操作计划的选择旨在补充现有的分拣机，并最大限度地使本申请中定义的研究人员以及UCSF的整个研究社区受益。

项目成果

The epigenetic regulator ATF7ip inhibits Il2 expression, regulating Th17 responses.

表观遗传调节因子 ATF7ip 抑制 Il2 表达，调节 Th17 反应。

• DOI: 10.1084/jem.20182316
• 发表时间: 2019
• 期刊: The Journal of experimental medicine
• 作者: Sin,JunHyung;Zuckerman,Cassandra;Cortez,JessicaT;Eckalbar,WalterL;Erle,DavidJ;Anderson,MarkS;Waterfield,MichaelR
• 通讯作者: Waterfield,MichaelR