The NINDS Human Cell and Data Repository

NINDS 人类细胞和数据存储库

• 批准号: 9150320
• 负责人: JAY Arnold TISCHFIELD
• 金额: $ 117.57万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9150320
• 关键词: Affect; Alzheimer' s Disease; Amyotrophic Lateral Sclerosis; Area; Automation; Biopsy; Blood; Blood Cells; Cataloging; Catalogs; Cell Line; Cells; Central Nervous System Diseases; Collaborations; Collecting Cell; Collection; Communities; DNA; Data; Databases; Derivation procedure; Development; Disease; Disease model; Dystonia; Emerging Technologies; Ensure; Ethics; Fibroblasts; Gene-Modified; Generations; Genomics; Goals; Housing; Human; Huntington Disease; Individual; Informatics; Instruction; International; Lymphocyte; Maintenance; Medicine; Methodology; Mission; Mutation; National Institute of Drug Abuse; National Institute of Mental Health; National Institute of Neurological Disorders and Stroke; Neurodegenerative Disorders; Neurons; Neurosciences; Online Systems; Paper; Parkinson Disease; Participant; Pharmaceutical Preparations; Play; Pluripotent Stem Cells; Positioning Attribute; Preclinical Drug Evaluation; Price; Process; Production; Protocols documentation; Publications; Quality Control; Recording of previous events; Regenerative Medicine; Reporting; Research; Research Personnel; Resources; Role; Sampling; Secure; Services; Shipping; Ships; Skin; Source; Specimen; Stem Cell Research; Stem cells; Technology; Testing; Therapeutic; Tissue Sample; Tissues; United States National Institutes of Health; Universities; Validation; Washington; Work; biobank; blastomere structure; cell registry; cell type; cost; design; exhibitions; falls; frontotemporal degeneration; genetic resource; genome editing; human disease; improved; induced pluripotent stem cell; interest; medical schools; meetings; nerve stem cell; news; novel; novel strategies; pluripotency; prospective; public health relevance; quality assurance; repaired; repository; research and development; sample collection; technology development; therapeutic development; tool; tool development; user-friendly; web portal; web site

 DESCRIPTION (provided by applicant): Human pluripotent stem cells that can give rise to almost any type of cell were originally isolated from embryonic cells and their utility as tools fo research and therapeutic development was severely restricted due to ethical considerations as well as the inability to derive stem cells from subjects with known disorders. The relatively recent development of technologies for deriving induced pluripotent stem cells (iPSCs) from cells that were previously thought to be "terminally" differentiated, such as skin or blood cells, has opened up nearly limitless new avenues for understanding human disease and developing novel approaches for treatment. By converting cells collected from affected individuals into iPSCs, and then to relevant cell types such as neurons for central nervous system disorders, we can create "disease models in a dish", cells that recapitulate the disorder and can be used to screen therapeutic drugs or study the manifestation of the disorder and elucidate its origins. The NINDS Repository was created to collect and distribute a variety of human cells such as skin fibroblasts and blood cells and convert these to iPSCs for research in the areas of neurodegenerative disease such as Parkinson's disease, Alzheimer's disease, Huntington's disease, amyotrophic lateral sclerosis, Dystonia and Frontotemporal Degeneration. Given the complexity and cost of generating iPSC lines of high quality, and the emerging importance of disease modeling and regenerative medicine, there is a tremendous need in the neuroscience field for a reliable centralized source of these materials. RUCDR Infinite Biologics (RUCDR) is uniquely positioned to provide this resource for the NINDS. RUCDR has a long and successful track record as a repository and genetic resource since its inception in 1998. RUCDR manages several major repositories for the NIH, including the NIMH Repository and Genomics Resource (NRGR) which encompasses the NIMH Stem Cell Resource. The mission of the RUCDR NINDS Stem Cell Repository will be to work with NINDS investigators and staff on all aspects of study management to collect, process and distribute the highest quality cell samples from participants in its studies. These will include the culturing of fibroblasts from skin biopsies, th isolation of lymphocytes from blood, and the derivation of iPSC lines from both cell types. RUCDR will then perform a comprehensive range of tests on the iPSCs to ensure their quality, pluripotency and identity. RUCDR will provide additional services to the global community, including the generation of neural stem cells (NSCs), different types of neurons, and genome editing to repair or introduce in iPSCs specific mutations that are implicated in human disease.

 描述（由适用提供）：几乎可以引起任何类型细胞的人多能干细胞从胚胎细胞中分离出来，最初是由于道德考虑以及无法从患有已知疾病的受试者的受试者衍生的干细胞而严重限制了作为FO研究和治疗性发育的工具。从以前被认为是“终止”分化的细胞（例如皮肤或血细胞）的细胞中诱导多能干细胞（IPSC）的技术相对发展，已经为了解人类疾病和开发新的治疗方法开辟了几乎有限的新途径。通过将收集的细胞从受影响的个体收集到IPSC中，然后转化为中枢神经系统疾病的神经元等相关细胞类型，我们可以创建“菜肴中的疾病模型”，这些细胞会概括该疾病，并可用于筛查治疗药物或研究该疾病的表现并阐明其起源。 Ninds存储库的创建是为了收集和分发各种人类细胞，例如皮肤成纤维细胞和血细胞，并将其转换为IPSC，以在神经退行性疾病的领域进行研究，例如帕金森氏病，阿尔茨海默氏病，亨廷顿氏病，亨廷顿氏病，杂种性的后期sclerlic sclerlic sclerisos，dystonia，dimynstonia and dystonia and tonstonia and interstonia和intrantol nemeneral anderatemal nemalitemal neryeral。鉴于产生高质量的IPSC线的复杂性和成本，以及疾病建模和再生医学的新兴重要性，在神经科学领域，对于这些材料的可靠集中式来源来说，有很大的需求。 RUCDR Infinite Biologics（RUCDR）具有独特的位置，可以为Ninds提供此资源。自1998年成立以来，RUCDR作为存储库和遗传资源具有长期而成功的记录。RUCDR管理了NIH的几个主要存储库，包括NIMH存储库和基因组学资源（NRGR），其中包括NIMH干细胞资源。 RUCDR NINDS干细胞存储库的任务将是与Ninds的研究人员和员工一起研究研究管理的各个方面，以收集，处理和分发其研究参与者的最高质量细胞样本。这些将包括从皮肤活检中培养成纤维细胞，从血液中分离淋巴细胞，以及两种细胞类型的IPSC系的衍生物。然后，RUCDR将对IPSC进行全面的测试，以确保其质量，多能性和身份。 RUCDR将为全球社区提供其他服务，包括神经元的产生（NSC），不同类型的神经元以及基因组编辑，以修复或在人类疾病中实施的IPSCS特定突变中进行介绍。