Rutgers University Cell and DNA Repository Renovation

罗格斯大学细胞和 DNA 储存库翻新

• 批准号: 7896252
• 负责人: JAY Arnold TISCHFIELD
• 金额: $ 949.21万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-04 至 2013-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7896252
• 关键词: Alcohol abuse; Alcoholism; Alcohols; Area; Arts; Autistic Disorder; Back; Biology; Bipolar Disorder; Blood specimen; Cells; Clinical; Complex; Continuing Education; Cryopreserved Cell; DNA; Diabetes Mellitus; Disease; Drug abuse; Employee; Epigenetic Process; Faculty; Floor; Funding; Future; Genetic; Human; Individual; Inflammatory Bowel Diseases; Institutes; Kidney Diseases; Laboratories; Liquid substance; Liver diseases; Mental Health; Molecular; Molecular Biology; Nicotine Dependence; Nitrogen; Nucleic Acids; Occupations; Process; Research; Research Personnel; Robotics; Sampling; Schizophrenia; Secure; Security; Services; Site; Stainless Steel; United States National Institutes of Health; Universities; Vacuum; Wing; Work; computerized data processing; design; improved; lymphoblastoid cell line; meetings; member; opioid abuse; repository; response; symposium

DESCRIPTION (provided by applicant): We request funds to renovate and improve existing spaces to provide lab and office areas for the Rutgers University Cell and DNA Repository (RUCDR), the designated core biosample processing and repository facility of four NIH Institutes; Alcohol and Alcohol Abuse; Digestive, Diabetes and Kidney Diseases; Drug Abuse; and Mental Health. RUCDR provides clinical biosample and data processing and varied repository services to more than 87 NIH-funded grantees researching complex diseases believed to have genetic or epigenetic etiological components, including autism, schizophrenia, bipolar disorder, opiate abuse, nicotine addiction, alcoholism, inflammatory bowel disease, liver disorders, and kidney diseases. RUCDR maintains more than 200,000 individual biosamples, most as lymphoblastoid cell lines established by the RUCDR from subject blood samples submitted by NIH researchers. Also, during the last 5 years, RUCDR has distributed more than 600,000 DNA samples to NIH-approved researchers. Several-fold increases in sample numbers from greater numbers of NIH projects that RUCDR must now support and the broadening of the scope of the state-of-the art molecular biology services that RUCDR now provides has created severe space shortages, infrastructural inadequacies, and sample security and workflow issues. In response to these challenges RUCDR has been forced to borrow existing spaces that are not well suited to RUCDR activities and that are dispersed over three different buildings. However, these spaces must be vacated for non-RUCDR uses in the near future. RUCDR now requires task-designed, easily accessible and contiguous laboratory space that does not currently exist within a single area. We propose renovation of the east end of the 2nd floor of C Wing, Nelson Biology Laboratories (NBL) to create facilities for automated (robotic) nucleic acid extraction, distribution and molecular analyses. The west end of the 2nd floor C Wing will provide interactive offices and work spaces for computational and statistical genetics faculty members affiliated with RUCDR and their research groups. Both areas are one floor directly above the existing RUCDR lab and administrative space in 1st floor C wing NBL. We also propose (1) additions to the Annex II building to produce a more secure and efficient back-up storage site for viable human cells cryopreserved in liquid nitrogen, (2) extension of the existing vacuum-jacketed, stainless steel liquid nitrogen delivery piping in Annex I to accommodate greater numbers of storage tanks and (3) expansion of the existing conference/continuing education room to accommodate daily task-oriented meetings of existing employees and new hires whose jobs will be created through this renovation.

描述（由申请人提供）：我们要求资金翻新和改善现有空间，以为罗格斯大学细胞和DNA存储库（RUCDR）提供实验室和办公区域，这是四个NIH Institutes的指定核心生物样品处理和库存库的指定的核心生物样品处理和存储库设施；酒精和酒精滥用；消化，糖尿病和肾脏疾病；吸毒；和心理健康。 RUCDR为87多名NIH资助的受赠人提供了临床生物样本和数据处理以及多样化的存储库服务，这些疾病研究的复杂疾病被认为具有遗传或表观遗传学的病因学成分，包括自闭症，精神分裂症，双皮亚疾病，双皮克障碍，阿片类药物滥用，鸦片滥用，尼古丁成瘾，酒精中毒，炎性肠疾病，Liver疾病，Liver Diseberes和sidgeys和kidneysease和kidneysease和sidgeysease和kidnedneys和kidneysease和kidnedney疾病。 RUCDR维持超过200,000个单个生物样本，大多数是由NIH研究人员提交的受试者血液样本建立的淋巴母细胞系。同样，在过去的5年中，RUCDR已向NIH批准的研究人员分发了600,000多个DNA样品。 RUCDR现在必须支持的大量NIH项目的样本数量增加了数量，RUCDR现在提供的最先进的分子生物学服务的范围扩大了严重的空间短缺，基础设施不足以及样本安全和工作流问题。为了应对这些挑战，RUCDR被迫借用不太适合RUCDR活动的现有空间，并且在三个不同的建筑物上分散。但是，这些空间必须在不久的将来腾出非RUCDR用途。 RUCDR现在需要任务设计，易于访问且可连续的实验室空间，这些空间当前不存在。我们建议对C机翼2楼的东端进行翻新，纳尔逊生物学实验室（NBL）为自动化（机器人）核酸提取，分布和分子分析的设施创建设施。二楼C翼的西端将为隶属于RUCDR及其研究小组的计算和统计遗传学教师提供互动办公室和工作空间。这两个区域都是直接在现有的RUCDR实验室和1楼C Wing NBL的管理空间上方的一层。我们还建议（1）在附件II建筑物中添加（1），以生成一个更安全，更有效的备份存储站点，以使可行的人类细胞在液氮中冷冻保存的可行细胞，（（2）现有的真空包装，不锈钢液体液体氮在附件I中的不锈钢液体氮的交付管道的扩展，以适应现有的储藏室和（3）室内的较大范围，以适应每日的教育，以适应室内的教育和（3）（3）（3）（3）（3）（3）（3）（3）（3）（3）（3）（3）（3）（3）（3）（3）（3）（3）雇用其工作将通过这种翻新创造的工作。