Rutgers University Cell and DNA Repository Renovation

罗格斯大学细胞和 DNA 储存库翻新

• 批准号: 7896252
• 负责人: JAY Arnold TISCHFIELD
• 金额: $ 949.21万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-04 至 2013-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7896252
• 关键词: Alcohol abuse; Alcoholism; Alcohols; Area; Arts; Autistic Disorder; Back; Biology; Bipolar Disorder; Blood specimen; Cells; Clinical; Complex; Continuing Education; Cryopreserved Cell; DNA; Diabetes Mellitus; Disease; Drug abuse; Employee; Epigenetic Process; Faculty; Floor; Funding; Future; Genetic; Human; Individual; Inflammatory Bowel Diseases; Institutes; Kidney Diseases; Laboratories; Liquid substance; Liver diseases; Mental Health; Molecular; Molecular Biology; Nicotine Dependence; Nitrogen; Nucleic Acids; Occupations; Process; Research; Research Personnel; Robotics; Sampling; Schizophrenia; Secure; Security; Services; Site; Stainless Steel; United States National Institutes of Health; Universities; Vacuum; Wing; Work; computerized data processing; design; improved; lymphoblastoid cell line; meetings; member; opioid abuse; repository; response; symposium

DESCRIPTION (provided by applicant): We request funds to renovate and improve existing spaces to provide lab and office areas for the Rutgers University Cell and DNA Repository (RUCDR), the designated core biosample processing and repository facility of four NIH Institutes; Alcohol and Alcohol Abuse; Digestive, Diabetes and Kidney Diseases; Drug Abuse; and Mental Health. RUCDR provides clinical biosample and data processing and varied repository services to more than 87 NIH-funded grantees researching complex diseases believed to have genetic or epigenetic etiological components, including autism, schizophrenia, bipolar disorder, opiate abuse, nicotine addiction, alcoholism, inflammatory bowel disease, liver disorders, and kidney diseases. RUCDR maintains more than 200,000 individual biosamples, most as lymphoblastoid cell lines established by the RUCDR from subject blood samples submitted by NIH researchers. Also, during the last 5 years, RUCDR has distributed more than 600,000 DNA samples to NIH-approved researchers. Several-fold increases in sample numbers from greater numbers of NIH projects that RUCDR must now support and the broadening of the scope of the state-of-the art molecular biology services that RUCDR now provides has created severe space shortages, infrastructural inadequacies, and sample security and workflow issues. In response to these challenges RUCDR has been forced to borrow existing spaces that are not well suited to RUCDR activities and that are dispersed over three different buildings. However, these spaces must be vacated for non-RUCDR uses in the near future. RUCDR now requires task-designed, easily accessible and contiguous laboratory space that does not currently exist within a single area. We propose renovation of the east end of the 2nd floor of C Wing, Nelson Biology Laboratories (NBL) to create facilities for automated (robotic) nucleic acid extraction, distribution and molecular analyses. The west end of the 2nd floor C Wing will provide interactive offices and work spaces for computational and statistical genetics faculty members affiliated with RUCDR and their research groups. Both areas are one floor directly above the existing RUCDR lab and administrative space in 1st floor C wing NBL. We also propose (1) additions to the Annex II building to produce a more secure and efficient back-up storage site for viable human cells cryopreserved in liquid nitrogen, (2) extension of the existing vacuum-jacketed, stainless steel liquid nitrogen delivery piping in Annex I to accommodate greater numbers of storage tanks and (3) expansion of the existing conference/continuing education room to accommodate daily task-oriented meetings of existing employees and new hires whose jobs will be created through this renovation.

描述(由申请人提供)：我们申请资金翻新和改善现有空间，为罗格斯大学细胞和DNA储存库(RUCDR)提供实验室和办公区域，RUCDR是四个NIH研究所指定的核心生物样本处理和储存库设施；酒精和酒精滥用；消化、糖尿病和肾脏疾病；药物滥用；以及精神健康。RUCDR为NIH资助的87多名受赠者提供临床生物样本和数据处理以及各种存储库服务，研究被认为具有遗传或表观遗传病因成分的复杂疾病，包括自闭症、精神分裂症、躁郁症、阿片剂滥用、尼古丁成瘾、酗酒、炎症性肠道疾病、肝脏疾病和肾脏疾病。RUCDR保存了20多万个单独的生物样本，其中大多数是RUCDR从NIH研究人员提交的受试者血液样本中建立的淋巴母细胞系。此外，在过去的5年里，RUCDR向NIH批准的研究人员分发了60多万个DNA样本。RUCDR现在必须支持的更多NIH项目的样本数量增加了几倍，RUCDR现在提供的最先进分子生物学服务的范围扩大，造成了严重的空间短缺、基础设施不足以及样本安全和工作流程问题。为了应对这些挑战，民盟被迫借用了不太适合民盟活动的现有空间，这些空间分散在三个不同的建筑中。然而，这些空间必须在不久的将来腾出用于非RUCDR用途。RUCDR现在需要任务设计的、容易进入的和连续的实验室空间，目前不存在于单一区域内。我们建议翻新纳尔逊生物实验室(NBL)C翼2楼东端，以创建自动化(机器人)核酸提取、分配和分子分析设施。二楼C翼的西端将为RUCDR附属的计算和统计遗传学教员及其研究小组提供互动办公室和工作空间。这两个区域都在现有RUCDR实验室和1楼C翼NBL行政空间的正上方一层。我们还建议(1)增加附件二大楼，为冷冻保存在液氮中的可存活的人体细胞提供一个更安全、更有效的后备储存场地；(2)扩建附件一现有的有真空夹套的不锈钢液氮输送管道，以容纳更多的储存罐；(3)扩建现有的会议/继续教育室，以容纳现有员工和新员工的日常会议，这些员工和新员工的工作将通过这次翻新而创造。