Rutgers University Cell and DNA Repository Renovation

罗格斯大学细胞和 DNA 储存库翻新

• 批准号: 7896252
• 负责人: JAY Arnold TISCHFIELD
• 金额: $ 949.21万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-04 至 2013-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7896252
• 关键词: Alcohol abuse; Alcoholism; Alcohols; Area; Arts; Autistic Disorder; Back; Biology; Bipolar Disorder; Blood specimen; Cells; Clinical; Complex; Continuing Education; Cryopreserved Cell; DNA; Diabetes Mellitus; Disease; Drug abuse; Employee; Epigenetic Process; Faculty; Floor; Funding; Future; Genetic; Human; Individual; Inflammatory Bowel Diseases; Institutes; Kidney Diseases; Laboratories; Liquid substance; Liver diseases; Mental Health; Molecular; Molecular Biology; Nicotine Dependence; Nitrogen; Nucleic Acids; Occupations; Process; Research; Research Personnel; Robotics; Sampling; Schizophrenia; Secure; Security; Services; Site; Stainless Steel; United States National Institutes of Health; Universities; Vacuum; Wing; Work; computerized data processing; design; improved; lymphoblastoid cell line; meetings; member; opioid abuse; repository; response; symposium

DESCRIPTION (provided by applicant): We request funds to renovate and improve existing spaces to provide lab and office areas for the Rutgers University Cell and DNA Repository (RUCDR), the designated core biosample processing and repository facility of four NIH Institutes; Alcohol and Alcohol Abuse; Digestive, Diabetes and Kidney Diseases; Drug Abuse; and Mental Health. RUCDR provides clinical biosample and data processing and varied repository services to more than 87 NIH-funded grantees researching complex diseases believed to have genetic or epigenetic etiological components, including autism, schizophrenia, bipolar disorder, opiate abuse, nicotine addiction, alcoholism, inflammatory bowel disease, liver disorders, and kidney diseases. RUCDR maintains more than 200,000 individual biosamples, most as lymphoblastoid cell lines established by the RUCDR from subject blood samples submitted by NIH researchers. Also, during the last 5 years, RUCDR has distributed more than 600,000 DNA samples to NIH-approved researchers. Several-fold increases in sample numbers from greater numbers of NIH projects that RUCDR must now support and the broadening of the scope of the state-of-the art molecular biology services that RUCDR now provides has created severe space shortages, infrastructural inadequacies, and sample security and workflow issues. In response to these challenges RUCDR has been forced to borrow existing spaces that are not well suited to RUCDR activities and that are dispersed over three different buildings. However, these spaces must be vacated for non-RUCDR uses in the near future. RUCDR now requires task-designed, easily accessible and contiguous laboratory space that does not currently exist within a single area. We propose renovation of the east end of the 2nd floor of C Wing, Nelson Biology Laboratories (NBL) to create facilities for automated (robotic) nucleic acid extraction, distribution and molecular analyses. The west end of the 2nd floor C Wing will provide interactive offices and work spaces for computational and statistical genetics faculty members affiliated with RUCDR and their research groups. Both areas are one floor directly above the existing RUCDR lab and administrative space in 1st floor C wing NBL. We also propose (1) additions to the Annex II building to produce a more secure and efficient back-up storage site for viable human cells cryopreserved in liquid nitrogen, (2) extension of the existing vacuum-jacketed, stainless steel liquid nitrogen delivery piping in Annex I to accommodate greater numbers of storage tanks and (3) expansion of the existing conference/continuing education room to accommodate daily task-oriented meetings of existing employees and new hires whose jobs will be created through this renovation.

描述（由申请人提供）：我们请求资金翻新和改善现有空间，为罗格斯大学细胞和 DNA 存储库 (RUCDR) 提供实验室和办公区域，RUCDR 是四个 NIH 研究所指定的核心生物样本处理和存储设施；酒精和酒精滥用；消化系统、糖尿病和肾脏疾病；药物滥用；和心理健康。 RUCDR 为超过 87 个 NIH 资助的受资助者提供临床生物样本和数据处理以及各种存储服务，研究被认为具有遗传或表观遗传病因的复杂疾病，包括自闭症、精神分裂症、双相情感障碍、阿片滥用、尼古丁成瘾、酗酒、炎症性肠病、肝脏疾病和肾脏疾病。 RUCDR 保存着超过 200,000 个个体生物样本，其中大部分是 RUCDR 从 NIH 研究人员提交的受试者血液样本中建立的类淋巴母细胞系。此外，在过去 5 年中，RUCDR 已向 NIH 批准的研究人员分发了超过 600,000 个 DNA 样本。 RUCDR 现在必须支持的 NIH 项目数量增加了数倍，并且 RUCDR 现在提供的最先进的分子生物学服务范围的扩大造成了严重的空间短缺、基础设施不足以及样本安全和工作流程问题。为了应对这些挑战，RUCDR 被迫借用现有空间，这些空间不太适合 RUCDR 活动，并且分散在三栋不同的建筑中。然而，这些空间必须在不久的将来腾出用于非 RUCDR 用途。 RUCDR 现在需要专门设计、易于访问且连续的实验室空间，而目前单个区域内并不存在这种空间。我们建议对尼尔森生物实验室 (NBL) C 翼二楼东端进行改造，以创建自动化（机器人）核酸提取、分配和分子分析设施。二楼 C 翼西端将为 RUCDR 及其研究小组的计算和统计遗传学教员提供互动办公室和工作空间。这两个区域均位于 NBL C 翼一楼现有 RUCDR 实验室和行政空间正上方的一层。我们还建议 (1) 扩建附件二大楼，为液氮中冷冻保存的活人类细胞提供一个更安全、更高效的备用储存场所，(2) 扩建附件一中现有的真空夹套不锈钢液氮输送管道，以容纳更多数量的储存罐，以及 (3) 扩建现有的会议/继续教育室，以容纳现有员工和新员工的日常任务导向会议。 通过这次改造将创造就业机会的员工。