Cell, Tissues, and Animal Core

说明)； 动物、细胞和组织培养核心(核心B)将负责提供 计划项目(PPG)包括实现特定目标所需的动物模型、组织和细胞。 这种PPG实验的重点是找出导致长寿差异的机制 啮齿动物的种类。啮齿动物为这种长寿的比较研究提供了一个独特的机会，因为 在一个单一的哺乳动物目中，寿命的差异超过10倍。例如，短- 活着的啮齿动物，如小鼠和大鼠，可以活3-5年，而长寿的啮齿动物，如裸鼠，是盲目的 鼹鼠、灰松鼠、海狸和豪猪的最长寿命从21岁到32岁不等。至 要研究这些动物长寿的分子机制，就必须提供 来自多种啮齿动物物种的几个个体的生物材料。核心B将保持和补充， 根据需要，收集18种啮齿动物的组织和原代细胞。核心将提供 对组织、细胞、DNA和RNA进行项目研究，并对细胞进行诱变处理。在……里面 加法。核心B将保留小鼠和裸鼠供项目调查人员使用。考虑到 从非商业用途的非标准生物中收集和建立细胞培养所需的努力 可用的，集中的资源将节省时间和精力，并降低成本！维护集中的 啮齿动物采集还将标准化培养条件、质量控制和使用的生物样本。 在PPG项目中，提高结果的重复性，并允许对相同个体动物的分析 通过几种化验和项目，以及最大限度地减少动物使用。 相关性(见说明)； 动物、细胞和组织培养核心(核心B)将维持动物、细胞和组织的收集 项目调查员。这一资源对于所有四个项目实现其具体目标至关重要。

instmctions); The Animal, Cell and Tissue Culture Core (Core B) will be responsible for providing investigators of the Program Project (PPG) with animal models, tissues and cells necessary to achieve their specific aims. Experiments of this PPG focus on identifying mechanisms responsible for differences in longevity between the species of rodents. Rodents offer a unique opportunity for such comparative study of longevity because of over 10-fold differences in life spans represented within a single mammalian order. For example, short- lived rodents, such as mouse and rat, live 3-5 years, while long-lived rodents, such as naked mole rat, blind mole rat, grey squirrel, beaver, and porcupine have maximum lifespans ranging from 21 to 32 years. To enable the study of molecular mechanisms of longevity in these animals, it is essential to provide access to biological materials from several individuals of multiple rodent species. Core B will maintain and replenish, as needed, the collection of tissues and primary cells from 18 species of rodents. The Core will provide project investigators with tissues, cells, DNA, and RNA, and perform mutagenic treatments of cells. In addition. Core B will maintain mice and naked mole rats for the use by project investigators. Considering the effort needed to collect and establish cell cultures from non-standard organisms that are not commercially available, the centralized resource will save time and effort, and reduce costs! Maintaining the centralized rodent collection will also standardize culture conditions, quality control and biological samples for use across PPG projects, improve reproducibility of results and allow the analysis of the same individual animals by several assays and projects, as well as minimize animal use. RELEVANCE (See instructions); The Animal, Cell and Tissue Culture Core (Core B) will maintain animal, cell and tissue collection for the project investigators. This resource is essential for all the four projects to achieve their specific aims.