Core B: Animal, Cell and Tissue Culture Core

核心 B：动物、细胞和组织培养核心

• 批准号: 10399518
• 负责人: Andrei Seluanov
• 金额: $ 45.56万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10399518
• 关键词: Age; Aging; Animal Model; Animals; Beavers; Biocompatible Materials; Biological; Biological Assay; Blood; Breeding; Cell Culture Techniques; Cell Line; Cell Separation; Cells; Collection; Comparative Study; DNA; DNA Damage; Data; Databases; Development; Engineering; Ensure; Epigenetic Process; Exhibits; Fibroblasts; Freezing; Gamma Rays; Gene Expression; Genes; Genome; Goals; HAS2 gene; Health; Hyaluronidase; IACUC; Individual; Intervention; Longevity; Maintenance; Malignant Neoplasms; Measures; Mole Rats; Molecular; Mus; Organism; Porcupines; Process; Protocols documentation; Quality Control; RNA; Rattus; Reproducibility of Results; Research Personnel; Resistance; Resources; Rodent; Role; Sampling; Squirrel; Standardization; Stress; Testing; Time; Tissue Banks; Tissue Harvesting; Tissues; Transgenic Mice; Wild Type Mouse; animal tissue; base; blind; comparative genomics; cost; epigenome; experimental study; gene therapy; improved; inhibitor; intervention effect; mouse model; overexpression; programs; tissue culture

SUMMARY: The Animal, Cell and Tissue Culture Core (Core B) is responsible for providing investigators of the Program Project (PPG) with animal models, tissues and cells necessary to achieve their specific aims. Experiments of this PPG focus on identifying mechanisms responsible for the differences in longevity between species of rodents. Rodents offer a unique opportunity for such comparative study of longevity because of the over 10-fold differences in lifespans represented within a single mammalian order. For example, short-lived rodents, such as the mouse and rat, live 3-5 years, while long-lived rodents, such as the naked mole rat, blind mole rat, grey squirrel, beaver, and porcupine have maximum lifespans ranging from 21 to 32 years. To enable the study of molecular mechanisms of longevity in these animals, it is essential to provide access to biological materials from several individuals of multiple rodent species. Core B maintains and replenishes, as needed, the collection of tissues and primary cells from over 22 species of rodents. The Core provides project investigators with tissues, cells, DNA and RNA, and performs stress treatments on animals. In addition, Core B will maintain mice, naked mole rats, blind mole rat and Damaraland mole rat colonies for the use of project investigators. Considering the effort needed to collect and establish cell cultures from non-standard organisms that are not commercially available, this centralized resource saves time and effort, and reduces costs. Core B will generate and breed mouse models with longevity interventions engineered based on the longevity mechanisms discovered by project investigators in long-lived rodent species. This includes mice overexpressing naked mole rat hyaluronan synthase 2 gene (nmrHAS2 mice), beaverized SIRT6 mice, and mice treated with hyaluronidase inhibitors. Core B will perform longevity studies with these mice and analyze the effect of interventions on health. Maintaining the centralized rodent collection standardizes culture conditions, quality control, and biological samples for use across PPG projects, improves reproducibility of results, and allows the analysis of the same individual animals by several assays and projects minimizing animal use.

总结：动物、细胞和组织培养核心（核心B）负责为研究者提供 计划项目（PPG）与动物模型，组织和细胞，以实现其特定的目标。 该PPG的实验集中于识别负责以下之间的寿命差异的机制： 啮齿动物的种类啮齿类动物为这种长寿的比较研究提供了一个独特的机会， 在同一个哺乳动物目中，寿命的差异超过10倍。例如，短暂的 啮齿类动物，如小鼠和大鼠，寿命3-5年，而长寿的啮齿类动物，如裸鼹鼠，失明 鼹鼠、灰松鼠、海狸和豪猪的最长寿命为21至32岁。使 在这些动物长寿的分子机制的研究，它是必不可少的，以提供生物 来自多个啮齿动物物种的几个个体的材料。核心B根据需要维护和更新 收集了超过22种啮齿动物的组织和原代细胞。核心组织提供项目调查人员 用组织、细胞、DNA和RNA，并对动物进行应激治疗。此外，核心B将保持 小鼠、裸鼹鼠、盲鼹鼠和达马拉岛鼹鼠群供项目调查人员使用。 考虑到从非标准微生物中收集和建立细胞培养物所需的努力， 这种集中的资源可以节省时间和精力，并降低成本。核心B将产生 并根据长寿机制设计长寿干预措施， 在长寿的啮齿类动物中发现的。这包括过度表达裸鼹鼠基因的小鼠 大鼠透明质酸合酶2基因（nmrHAS 2小鼠）、海狸化SIRT 6小鼠和用透明质酸酶处理的小鼠 抑制剂的核心B将对这些小鼠进行长寿研究，并分析干预措施对健康的影响。 保持集中的啮齿动物收集， 用于PPG项目的样品，提高了结果的再现性，并允许对相同的结果进行分析。 个体动物通过几个试验和项目最大限度地减少动物使用。