Core B: Animal, Cell and Tissue Culture Core

摘要：动物、细胞和组织培养核心(核心B)负责为 计划项目(PPG)包括实现特定目标所需的动物模型、组织和细胞。 这种PPG实验的重点是确定导致长寿差异的机制 啮齿动物的种类。啮齿动物为这种长寿的比较研究提供了一个独特的机会，因为 单个哺乳动物的寿命差异超过10倍。例如，短暂的 啮齿动物，如老鼠和大鼠，可以活3-5年，而长寿的啮齿动物，如裸鼠，则失明 鼹鼠、灰松鼠、海狸和豪猪的最长寿命从21岁到32岁不等。要启用 研究这些动物长寿的分子机制，对于提供生物学途径是至关重要的 材料来自多种啮齿动物的几个个体。核心B根据需要维护和补充 收集了超过22种啮齿动物的组织和原代细胞。核心为项目调查员提供 研究组织、细胞、DNA和RNA，并对动物进行应激治疗。此外，核心B将保持 小鼠、裸鼠、盲鼠和达马拉鼹鼠群体供项目调查人员使用。 考虑到从非标准生物中收集和建立细胞培养物所需的努力 商业上可以买到，这种集中的资源节省了时间和精力，并降低了成本。核心B将生成 并用基于长寿机制设计的长寿干预措施培育小鼠模型 由项目调查人员在长寿啮齿动物物种中发现。这包括过度表达裸露痣的小鼠 大鼠透明质酸合成酶2基因(nmrHAS2小鼠)、SIRT6小鼠和透明质酸酶处理小鼠 抑制剂。Core B将对这些小鼠进行长寿研究，并分析干预措施对健康的影响。 集中收集啮齿动物可规范养殖条件、质量控制和生物 在PPG项目中使用的样本，提高了结果的重现性，并允许对结果进行分析 通过几种化验和项目将动物使用量降至最低。

SUMMARY: The Animal, Cell and Tissue Culture Core (Core B) is responsible for providing investigators of the Program Project (PPG) with animal models, tissues and cells necessary to achieve their specific aims. Experiments of this PPG focus on identifying mechanisms responsible for the differences in longevity between species of rodents. Rodents offer a unique opportunity for such comparative study of longevity because of the over 10-fold differences in lifespans represented within a single mammalian order. For example, short-lived rodents, such as the mouse and rat, live 3-5 years, while long-lived rodents, such as the naked mole rat, blind mole rat, grey squirrel, beaver, and porcupine have maximum lifespans ranging from 21 to 32 years. To enable the study of molecular mechanisms of longevity in these animals, it is essential to provide access to biological materials from several individuals of multiple rodent species. Core B maintains and replenishes, as needed, the collection of tissues and primary cells from over 22 species of rodents. The Core provides project investigators with tissues, cells, DNA and RNA, and performs stress treatments on animals. In addition, Core B will maintain mice, naked mole rats, blind mole rat and Damaraland mole rat colonies for the use of project investigators. Considering the effort needed to collect and establish cell cultures from non-standard organisms that are not commercially available, this centralized resource saves time and effort, and reduces costs. Core B will generate and breed mouse models with longevity interventions engineered based on the longevity mechanisms discovered by project investigators in long-lived rodent species. This includes mice overexpressing naked mole rat hyaluronan synthase 2 gene (nmrHAS2 mice), beaverized SIRT6 mice, and mice treated with hyaluronidase inhibitors. Core B will perform longevity studies with these mice and analyze the effect of interventions on health. Maintaining the centralized rodent collection standardizes culture conditions, quality control, and biological samples for use across PPG projects, improves reproducibility of results, and allows the analysis of the same individual animals by several assays and projects minimizing animal use.