Core B: Animal, Cell and Tissue Culture Core

摘要：动物、细胞和组织培养核心（核心 B）负责为研究人员提供 计划项目（PPG），包含实现其特定目标所需的动物模型、组织和细胞。 该 PPG 的实验重点是确定导致不同物种寿命差异的机制。 啮齿类动物的种类。啮齿类动物为这种长寿比较研究提供了独特的机会，因为 同一哺乳动物目中的寿命差异超过 10 倍。例如，短暂的 啮齿类动物，如小鼠和大鼠，寿命为 3-5 年，而长寿的啮齿类动物，如裸鼹鼠，则失明 鼹鼠、灰松鼠、海狸和豪猪的最长寿命为 21 至 32 年。启用 研究这些动物长寿的分子机制，必须提供生物信息 来自多种啮齿动物物种的多个个体的材料。核心 B 根据需要维护和补充 收集超过 22 种啮齿动物的组织和原代细胞。核心提供项目调查员 与组织、细胞、DNA 和 RNA 结合，并对动物进行应激治疗。此外，Core B将维持 供项目研究人员使用的小鼠、裸鼹鼠、盲鼹鼠和达马拉兰鼹鼠群体。 考虑到从非标准生物体收集和建立细胞培养物所需的努力 这种集中式资源可在商业上使用，可以节省时间和精力，并降低成本。核心B将生成 并培育具有基于长寿机制的长寿干预措施的小鼠模型 项目研究人员在长寿的啮齿动物物种中发现了这一点。这包括过度表达裸鼹鼠的小鼠 大鼠透明质酸合酶 2 基因（nmrHAS2 小鼠）、海狸化 SIRT6 小鼠和用透明质酸酶处理的小鼠 抑制剂。 Core B 将利用这些小鼠进行长寿研究，并分析干预措施对健康的影响。 维持集中的啮齿动物收集可以标准化培养条件、质量控制和生物学 跨 PPG 项目使用的样本，提高结果的可重复性，并允许对相同的结果进行分析 通过多种分析和项目对单个动物进行最小化动物使用。

SUMMARY: The Animal, Cell and Tissue Culture Core (Core B) is responsible for providing investigators of the Program Project (PPG) with animal models, tissues and cells necessary to achieve their specific aims. Experiments of this PPG focus on identifying mechanisms responsible for the differences in longevity between species of rodents. Rodents offer a unique opportunity for such comparative study of longevity because of the over 10-fold differences in lifespans represented within a single mammalian order. For example, short-lived rodents, such as the mouse and rat, live 3-5 years, while long-lived rodents, such as the naked mole rat, blind mole rat, grey squirrel, beaver, and porcupine have maximum lifespans ranging from 21 to 32 years. To enable the study of molecular mechanisms of longevity in these animals, it is essential to provide access to biological materials from several individuals of multiple rodent species. Core B maintains and replenishes, as needed, the collection of tissues and primary cells from over 22 species of rodents. The Core provides project investigators with tissues, cells, DNA and RNA, and performs stress treatments on animals. In addition, Core B will maintain mice, naked mole rats, blind mole rat and Damaraland mole rat colonies for the use of project investigators. Considering the effort needed to collect and establish cell cultures from non-standard organisms that are not commercially available, this centralized resource saves time and effort, and reduces costs. Core B will generate and breed mouse models with longevity interventions engineered based on the longevity mechanisms discovered by project investigators in long-lived rodent species. This includes mice overexpressing naked mole rat hyaluronan synthase 2 gene (nmrHAS2 mice), beaverized SIRT6 mice, and mice treated with hyaluronidase inhibitors. Core B will perform longevity studies with these mice and analyze the effect of interventions on health. Maintaining the centralized rodent collection standardizes culture conditions, quality control, and biological samples for use across PPG projects, improves reproducibility of results, and allows the analysis of the same individual animals by several assays and projects minimizing animal use.