Identification of stretch-induced biotinylation at cadherin junctions

钙粘蛋白连接处拉伸诱导的生物素化的鉴定

• 批准号: 9182555
• 负责人: Soichiro Yamada
• 金额: $ 7.08万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2018-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9182555
• 关键词: Actins; Adhesions; Adhesives; Area; Biochemical; Biochemistry; Biotin; Biotinylation; Cadherins; Cell Communication; Cell Count; Cell-Cell Adhesion; Cells; Cellular Morphology; Complex; Cultured Cells; Custom; Cytoskeleton; Data; Detection; Devices; Embryonic Development; Frequencies; Goals; Home environment; In Situ; In Vitro; Individual; Intercellular Junctions; Label; Lavandula; Length; Ligase; Malignant Epithelial Cell; Mass Spectrum Analysis; Mechanical Stimulation; Mechanics; Membrane; Molecular; Motor; Neoplasm Metastasis; Nonmuscle Myosin Type IIA; Organ; Performance; Phenotype; Positioning Attribute; Precipitation; Property; Proteins; Proteomics; Protocols documentation; Regulation; Role; Sampling; Silicon; Silver Staining; Slide; Staging; Streptavidin; Stretching; Techniques; Tissues; Variant; Western Blotting; Wound Healing; alpha catenin; base; cell motility; design; flexibility; innovation; new therapeutic target; programs; protein complex; protein profiling; protein protein interaction; prototype; scale up; screening

Project Summary During embryogenesis or wound healing, neighboring cells maintain contact and migrate collectively, though the roles of cell-cell adhesion during collective cell migration is poorly defined. Due to constant pulling and pushing between migrating neighboring cells, we hypothesize that mechanical forces regulate the interaction between the cell-cell adhesion complex and the actin cytoskeleton, and therefore, the adhesive strength. To identify force-sensitive protein complexes at cell-cell junctions, our innovative biochemical analysis combines in situ proximal biotin labeling with a cell stretch device that promotes the formation of force-sensitive complexes. By fusing α-catenin with a promiscuous biotin ligase, any proximal proteins of α-catenin will be biotinylated. The force-dependent change in the biotinylation profile is an indication of altered protein complexes. Our preliminary study demonstrates that α-catenin and myosin IIA are likely interacting in a force-dependent manner. While the current approach is suited for the candidate screening of force-sensitive proteins, the application of proteomic screening to this approach will be transformative, because the proteomic screening will reveal the total composition of α-catenin associated proteins in the presence or absence of external forces, a critical first step in deciphering the molecular basis of mechano- transduction. However, the key limitation of the current protocol is that the small cell stretch chambers that limit the quantity of protein samples. Our goal of this proposal is to re-design and scale-up the current protocol to isolate the quantity of purified proteins sufficient for mass spectrometry and identify the force-sensitive complex surrounding α-catenin. We will fabricate cell stretch chambers based on a silicon sheet as a substrate to culture cells. Using this device, we will optimize the mechanical stimulation (the frequency, the magnitude and the duration of substrate stretch) based on cell morphology, the organization of the actin cytoskeleton, and the extent of biotinylation. Using the newly designed cell stretcher and mass spectrometry analysis, we will determine a comprehensive list of the force-sensitive molecules surrounding α- catenin that is essential for understanding of mechano-transduction.

项目总结