Gene Library Resource for the Sea Urchin S. purpuratus

海胆 S. purpuratus 基因库资源

• 批准号: 9320317
• 负责人: Robert Andrew Cameron
• 金额: $ 18.95万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9320317
• 关键词: Animals; Asterina; Bacterial Artificial Chromosomes; Binding; Biological; Biological Models; Chromosome Mapping; Collaborations; Communities; Comparative Study; Consultations; Custom; DNA; DNA Sequence; Data; Data Set; Databases; Development; Developmental Cell Biology; Developmental Gene; Distant; Embryo; Engineering; Gene Expression; Gene Library; Gene Transfer; Gene Transfer Techniques; Genes; Genome; Genomic DNA; Genomics; Gravidity; Hand; Head; Health; Health Services Research; In Vitro; Knowledge; Laboratories; Laboratory Animals; Libraries; Lytechinus; Maintenance; Measurement; Messenger RNA; Methods; Modeling; Molecular Biology; Mosaicism; Peptides; Preparation; Proteomics; RNA; Reagent; Recombinants; Regulator Genes; Research; Research Infrastructure; Resources; Sea Urchins; Sequence Analysis; Services; Source; Starfish; Strongylocentrotus purpuratus; Structure; Synthetic Genes; System; Technology; base; college; data structure; deep sequencing; design; experience; falls; genome sequencing; human genome sequencing; improved; instrumentation; medical schools; nano-string; new technology; next generation; operation; research study; transcription factor; transcriptome; web site

DESCRIPTION (provided by applicant): This revised proposal seeks support for the Gene Library Resource for the purple sea urchin, Strongylocentrotus purpuratus. This Resource provides the essential infrastructure, new technology, molecular biology support services, and both material and informational resources for the whole field of sea urchin research. The Resource is unique in the world, and it is fair to state that directly or indirectly it has impacted every sea urchin laboratory in the US and elsewhere as well. In the last five years the P40 provided essential technological and material support that made it possible for HGRI to obtain the genomic sequence of S. purpuratus. This animal has become a major research model, and knowledge of the genomically encoded regulatory networks underlying development of its embryo is more advanced than for any other animal system. Recently, HGRI approved support for sequencing of several further echinoderm species (at the Human Genome Sequencing Center, Baylor College School of Medicine). This decision was taken because of the major scientific advantages that would be available for the research community were the genomes of these additional echinoderm species for comparative studies of gene regulatory network organization, for cis-regulatory structure-function studies, and for research on mechanisms of embryonic and postembryonic development. But research model systems are useful in these fields only if the biological parameters of operation in the system as well as the sequence of the genome are in hand. The service and research objectives of the present renewal application thus fall into two broad components: to further support and develop the S. purpuratus research resource, by provision of advanced technologies and databases, and to establish equivalent technological and biological as well as material resources for the additional echinoderm species, while providing essential support for the genome sequencing efforts now being directed at these species. The initial set of additional species with which we are here concerned, all excellent laboratory animals, are a sea star, Asterina miniata, another very widely used sea urchin, Lytechinus varieaatus, and a distantly diverged echinoid, Eucidaris tribuloides.

描述（由申请人提供）：本修订提案寻求对紫海胆（Strongylocentrotus purpuratus）基因库资源的支持。该资源为整个海胆研究领域提供了必要的基础设施，新技术，分子生物学支持服务以及材料和信息资源。该资源在世界上是独一无二的，公平地说，它直接或间接地影响了美国和其他地方的每个海胆实验室。在过去的五年里，P40提供了必要的技术和物质支持，使HGRI获得了S.紫色的这种动物已经成为一个主要的研究模型，其胚胎发育背后的基因组编码调控网络的知识比任何其他动物系统都要先进。最近，HGRI批准了对其他几种棘皮动物物种的测序支持（在贝勒医学院人类基因组测序中心）。之所以做出这一决定，是因为研究界可以利用这些额外棘皮动物物种的基因组进行基因调控网络组织的比较研究，顺式调控结构-功能研究以及胚胎和胚后发育机制的研究。但是，研究模型系统只有在系统中的生物学操作参数以及基因组序列在手的情况下才能在这些领域中有用。因此，本更新申请的服务和研究目标分为两大部分：进一步支持和发展S。通过提供先进的技术和数据库，加强棘皮动物研究资源，并为其他棘皮动物物种建立同等的技术、生物和物质资源，同时为目前针对这些物种的基因组测序工作提供必要的支持。我们在这里所涉及的最初一组其他物种，都是优秀的实验室动物，是星星，Asterina miniata，另一种非常广泛使用的海胆，Lytechinus varieaatus，以及一种远距离分叉的海胆，Eucidaris tribuloides。