Study on the Avoidance Response in Asterina pectinifera to Solaster dawsoni
海燕对道索尼叶甲的回避反应研究
基本信息
- 批准号:10460094
- 负责人:
- 金额:$ 4.29万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Asterina pectinifera flees from the predator asteroid Solaster dawsoni before contact. This response is called an avoidance reaction. A. pectinifera is the only asteroid among marine fauna, which show the avoidance reaction. Several asteroids, such as Asterias rubens, show an escape response, which refer to the physical contact by the opponent to reject the predation.We have elucidated that the avoidance response in A. pectinifera is induced by biomolecules of S. dawsoni, and in the present research project, we aimed to develop a quantitative bioassay and isolation and purification of bioactive compounds responsible to the avoidance reaction.The bioactivity was quantified by measuring the following items : "lag time before avoidance response", "average speed of evasion", and "distance from the origin". Each item was evaluated by five grades, and the sum of grades of three items were categorized into four intensities of bioactivity (+++, ++, +, and -). A liquid (drip) obtained from S. dawsoni by freeze-thaw procedure elicited the strongest response (+++). The bioactivity of the drip was eliminated by Pronase E treatment. Bioactive substances were remained in the inner solution by the ultra filtration of 30,000. Separation by ammonium sulfate fractionation, gel filtration chromatography, and HPLC revealed that the avoidance response in A. pectinifera elicited by several proteinaceous compounds with molecular weight of around 100,000. As far as we can tell that this is the first report of allelochemicals having such larger molecular weights.In the course of the above study, we found that the aquarium water of A. pectinifera showed remedial and preventive effects on scaly area of human hands. Two polyhydroxysteroids and three steroidal saponins (pectiniosides A, B and C) were isolated as bioactive substances from the water extract of A. pectinifera.
Asterina pectinifera在接触前逃离掠食者小行星Solaster dawsoni。这种反应被称为回避反应。在海洋动物群中,只有A. pectinifera表现出回避反应。一些小行星,如阿斯特里亚斯鲁本斯,表现出逃避反应,这是指对手的身体接触,以拒绝捕食。我们已经阐明了果胶的回避反应是由S. dawsoni的生物分子诱导的,在本研究项目中,我们旨在建立定量的生物测定和分离纯化与回避反应有关的生物活性化合物。生物活性通过测量“躲避反应前的滞后时间”、“平均躲避速度”和“离原点的距离”来量化。每个项目分为五个等级进行评价,三个项目的等级之和分为四个生物活性强度(+++,++,+,-)。冻融法提取的dawsoni液体(滴液)反应最强(+++)。Pronase E处理消除了滴注的生物活性。生物活性物质通过30000级超滤保留在内液中。通过硫酸铵分馏法、凝胶过滤色谱法和高效液相色谱法分离发现,果胶中有几种分子量在10万左右的蛋白类化合物引起回避反应。据我们所知,这是首次报道具有如此大分子量的化感物质。在上述研究过程中,我们发现果胶刺针水族水对人手鳞片区有治疗和预防作用。从果胶水提物中分离得到两种多羟基类固醇和三种甾体皂苷(果胶苷A、B和C)。
项目成果
期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kazuyo Ukai, et al.: "Prevention by Polyhydroxysteroids and Saponins of Asterina pectinifera of the Desquamation of Stratum Comeum Cells"Biosci. Biotech. Biochem.. 66巻. 913-915 (2002)
Kazuyo Ukai 等人:“海燕多羟基类固醇和皂苷预防角质层细胞脱皮”Biosci. Biochem. 66. 913-915 (2002)
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Kazuyo Ukai, Shinji Kirihara, Yoshikazu Fujikawa, Masahiro Notoya, and Michio Namikoshi: "Identification of Two Nucleosides, Inosine and Guanosine, in the bioactive Fraction from Solaster dawsoni that Induced Escape Response in Asterina pectinifera"J. Tok
Kazuyo Ukai、Shinji Kirihara、Yoshikazu Fujikawa、Masahiro Notoya 和 Michio Namikoshi:“从海藻中诱导逃避反应的道索尼生物活性组分中两种核苷(肌苷和鸟苷)的鉴定”J.
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Kazuyo Ukai, et al.: "Identification of Two Nucleosides, Inosine and Guanosine, in the Bioactive Fraction from Solaster dawsoni that Induced Escape Response in Asterina pectinifera"J. Tokyo Univ. Fisheries. 88巻. 7-13 (2002)
Kazuyo Ukai 等人:“从海藻中诱导逃逸反应的道索尼生物活性组分中肌苷和鸟苷的鉴定”J. Tokyo Univ. Vol. 88. 7-13 (2002)。
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Kazuyo Ukai, Atsufumi Iwanaga, Hiroshi Nagai, and Michio Namikoshi: "Prevention by Polyhydroxysteroids and Saponins of Asterina pectinifera of the Desquamation of Stratum Comeum Cells"Biosci. Biotechnol. Biochem.. 66(4). 913-915 (2002)
Kazuyo Ukai、Atsufumi Iwanaga、Hiroshi Nagai 和 Michio Namikoshi:“通过多羟基类固醇和海燕皂苷预防角质层细胞脱皮”Biosci。
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Kazuyo Ukai, et al.: "Prevention by Polyhydroxysteroids and Saponins of Asterina pectinifera of the Desquamation of Stratum Comeum Cells"Biosci. Biotech. Biochem.. 66(印刷中). (2002)
Kazuyo Ukai 等人:“海燕多羟基类固醇和皂苷预防角质层细胞脱皮”Biosci.Biochem.66(出版中)。
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NAMIKOSHI Michio其他文献
NAMIKOSHI Michio的其他文献
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{{ truncateString('NAMIKOSHI Michio', 18)}}的其他基金
Studies on the mechanisms of anticancer and anti-inflammatory activities of alkaloids from ascidians
海鞘生物碱抗癌、抗炎作用机制研究
- 批准号:
21603012 - 财政年份:2009
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Studies on the Structure and Function of Autotomy Promoting Factor in Sea Stars
海星自残促进因子的结构与功能研究
- 批准号:
15380140 - 财政年份:2003
- 资助金额:
$ 4.29万 - 项目类别:
Grant-in-Aid for Scientific Research (B)