Investigating the extracellular matrix in vascular development and maintenance

研究细胞外基质在血管发育和维持中的作用

基本信息

批准号：
9087349
负责人：
Douglas Gould
金额：
$ 34.67万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2018-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9087349
关键词：
Acids Address Affect American Heart Association Basement membrane Binding Biochemical Blood Vessels Brain hemorrhage Caenorhabditis elegans Cause of Death Cells Cerebral hemisphere hemorrhage Cerebrovascular Disorders Cerebrum Cessation of life Chemicals Child Code Collagen Type IV Data Defect Deposition Development Disease Etiology Event Extracellular Matrix FDA approved Gene Expression Profiling Genes Genetic Genetic Models Genetic study Health Homeostasis Human In Vitro Inherited Intervention Intracranial Aneurysm Lead Lesion Leukoencephalopathy Maintenance Membrane Proteins Molecular Molecular Chaperones Molecular Genetics Mus Mutant Strains Mice Mutation Pathogenesis Pathology Pathway interactions Patients Pericytes Perinatal Perinatal subependymal hemorrhage Play Pre-Clinical Model Prevention Preventive therapy Proteins Regulation Reporting Research Proposals Risk Role Series Severities Signal Pathway Signal Transduction Signal Transduction Pathway Stroke Stroke prevention System Testing Therapeutic Transforming Growth Factor beta United States Vascular Diseases Vascular Endothelial Cell Work age related aged angiogenesis base brain endothelial cell cell type cerebral microbleeds cerebrovascular cerebrovascular lesion conditional mutant disability effective therapy efficacy testing extracellular human disease in vivo juvenile animal member mouse model mutant novel paralogous gene patient subsets pre-clinical prevent protein folding targeted treatment young adult

项目摘要

DESCRIPTION (provided by applicant): Intracerebral hemorrhages (ICH) and hemorrhagic stroke are a particularly fatal form of stroke and an important cause of long-term disability. This is especially true when they affect children and young adults. Effective treatments for hemorrhagic stroke are limited, and so ICH prevention is paramount for reducing the impact of this debilitating condition. However, preventative therapies often require an understanding of the pathogenic mechanism underlying ICH. Genetic studies have successfully defined the etiologies of some forms of ICH, however there is still a tremendous unmet need in determining the underlying causes of most forms of CVD. We have discovered that mutations in type IV collagen alpha 1 (COL4A1) cause a broad spectrum of highly penetrant cerebrovascular diseases (CVDs), including peri-natal stroke, congenital porencephaly, leukoencephalopathy, cerebral microbleeds, intracranial aneurysm, and ICH. In addition to multiple reports of COL4A1 mutations causing dominantly inherited CVD, our recent data suggest that 5-10% of patients suffering from sporadic ICHs have mutations in COL4A1 or its paralog COL4A2. Thus, according to American Heart Association estimates, mutations in COL4A1 and COL4A2 could cause up to 12,000 new cases of spontaneous ICH in the United States each year and 300,000 cases worldwide. Here we use novel genetic models of CVD to identify distinct roles for COL4A1 in vascular development and maintenance. Using genetic, molecular, and biochemical approaches we will directly test the regulation by COL4A1 of fundamental cell- signaling pathways that are critical for normal vascular development. Finally, we will use novel pre-clinical models of human CVD that we have generated to identify therapeutics that may be developed for use in patients and that may reduce their risk of suffering debilitating or fatal ICHs.

描述（由申请人提供）：脑内出血（ICH）和出血性中风是中风的一种特别致命的形式，也是长期残疾的重要原因。这当他们影响儿童和年轻人时，尤其如此。出血性中风的有效治疗是有限的，因此预防ICH对于减少这种衰弱状况的影响至关重要。但是，预防疗法通常需要了解ICH基础的致病机制。遗传研究成功地定义了某些形式的ICH的病因，但是在确定大多数形式的CVD的根本原因方面仍然存在巨大的未满足需求。我们发现，IV型胶原蛋白α1（COL4A1）中的突变引起了广泛的高度渗透性脑脑疾病（CVD），包括围场性 - 纳塔骨骼，先天性孔内脑，白细胞畸形，白细胞畸形，脑脑膜，脑膜，内脑静脉内脑膜和iChich。除了对COL4A1突变的多次报道引起了主要遗传CVD，我们最近的数据表明，患有零星ICH的患者中有5-10％的患者在COL4A1或其旁系同源物COL4A2中具有突变。因此，根据美国心脏协会的估计，COL4A1和COL4A2的突变每年可能会导致多达12,000例新的自发ICH案例，全球30万例病例。在这里，我们使用CVD的新型遗传模型来确定COL4A1在血管发育和维持中的不同作用。使用遗传，分子和生化方法，我们将通过COL4A1直接测试对正常血管发育至关重要的基本细胞信号通路的调节。最后，我们将使用新颖的临床前我们已经生成的人类CVD模型来鉴定可能用于患者使用的治疗剂，并可能降低其遭受衰弱或致命的ICH的风险。