Core A - Animal core

项目总结/摘要 使用转基因小鼠已经彻底改变了许多复杂的生物过程的研究 包括免疫耐受和自身免疫。这一点在本PPG的所有项目中都得到了明确反映， 严重依赖转基因、基因敲除和自身免疫易感小鼠品系的使用。一只老鼠的核心是 这将有助于简化和提高效率， 在健康无病原体的环境中培育基因复杂的品系。 该核心的具体目标是： 1.对所有项目使用的独特和非常规小鼠品系进行繁殖和遗传表征 项目成员。 2.协助项目成员对新获得或衍生的菌株进行常规回交， 计划研究的适当遗传背景 3.确保提供给项目成员的动物是健康的，并保持在无病原体的环境中， 为研究提供环境。 核心的可用性确保PPG研究者能够获得健康和无病原体的 具有复杂遗传组成的动物用于拟议的研究。

Project Summary/Abstract The use of genetically altered mice has revolutionized the study of many complex biological processes including immune tolerance and autoimmunity. This is clearly reflected in all the projects in this PPG, which rely heavily on the use of transgenic, knockout, and autoimmune-prone mouse strains. A mouse Core is envisioned that will help streamline and improve efficiencies with the generation and maintenance of genetically complex lines in a healthy, pathogen-free environment. The specific aims of this Core are: 1. To breed and genetically characterize unique and non-routine mouse strains for use by all of the program project members. 2. To assist program project members with routine backcrossing of newly obtained or derived strains to the appropriate genetic backgrounds for planned studies 3. To ensure animals provided to program project members are healthy and maintained in a pathogen-free environment for the proposed studies. The availability of the Core ensures that the PPG investigators will be able to obtain healthy and pathogen-free animals of complex genetic make-up for use in the proposed studies.