Core A - Animal core

项目摘要/摘要 转基因小鼠的使用彻底改变了对许多复杂生物过程的研究。 包括免疫耐受和自身免疫。这一点清楚地反映在这个PPG的所有项目中，这些项目 严重依赖转基因、基因敲除和有自身免疫倾向的小鼠品系的使用。鼠标核心是 预计这将有助于简化和提高效率，从而生成和维护 在健康、无病原体的环境中培育基因复杂的品系。 这一核心的具体目标是： 1.培育独特的和非常规的小鼠品系，并对其进行基因鉴定，以供所有计划使用 项目成员。 2.协助计划项目成员将新获得或派生的菌株常规回交到 为计划的研究提供适当的遗传背景 3.确保提供给计划项目成员的动物健康并保持在无病原体的环境中 建议进行研究的环境。 核心的可用性确保PPG调查人员将能够获得健康和无病原体的 用于拟议研究的复杂遗传构成的动物。

Project Summary/Abstract The use of genetically altered mice has revolutionized the study of many complex biological processes including immune tolerance and autoimmunity. This is clearly reflected in all the projects in this PPG, which rely heavily on the use of transgenic, knockout, and autoimmune-prone mouse strains. A mouse Core is envisioned that will help streamline and improve efficiencies with the generation and maintenance of genetically complex lines in a healthy, pathogen-free environment. The specific aims of this Core are: 1. To breed and genetically characterize unique and non-routine mouse strains for use by all of the program project members. 2. To assist program project members with routine backcrossing of newly obtained or derived strains to the appropriate genetic backgrounds for planned studies 3. To ensure animals provided to program project members are healthy and maintained in a pathogen-free environment for the proposed studies. The availability of the Core ensures that the PPG investigators will be able to obtain healthy and pathogen-free animals of complex genetic make-up for use in the proposed studies.