Ultramicrotome

 描述（由申请人提供）：我们申请资金购买Leica EM UC 7超薄切片机，用于在室温下切片生物和生物材料样品。新仪器将有助于满足马萨诸塞州大学医学院（UMMS）和生物医学科学研究生院（GSBS）60多个用户实验室的超薄切片需求。新的超薄切片机将被安置在医学院的核心电子显微镜设施中，并将取代30岁的Reichert-Jung Ultracut E，该设备虽然仍在运行，但现在被认为是过时的，已经变得不可靠，并且不再得到制造商的支持（零件或服务）。 超微切片机用于切割细胞和组织的薄切片，这是了解其详细结构和人类疾病背后的结构变化所必需的。许多组织工程策略涉及通过多孔聚合物支架递送细胞。获得新生组织的切片对于分析微观环境的特征是必要的。核心电子显微镜设施支持的项目范围从肌肉结构，微管形成和稳定，干细胞和生殖细胞分化，纤毛和鞭毛结构的研究，仅举几例。所有这些都是了解人体正常功能和结构的重要课题，都需要超微切片术。 Leica EM UC 7超薄切片机与我们的老款Reichert-Jung Ultracut E型号相比，具有一系列出色的功能和优势。每个用户，从绝对的初学者学生到我们最熟练的超微切片机将受益于这一现代仪器。观察系统的同心运动允许即使在水位降低的情况下也能检查切片（如免疫标记项目中使用的Lowycryl和LR白色树脂）。Leica EM UC 7的触摸感应控制单元可通过帮助文件和提示快速安全地对刀和样本进行对准，简化了修剪过程，同时为切片准备样本。 资助这笔赠款将帮助我们为我们的共享研究电子显微镜核心设施提供最先进的仪器，由100多名研究人员及其研究生以及周边地区几所大学的教师和研究生使用。新仪器将大大提高我们的研究人员和学生进行显微切片相关研究的能力，它将大大提高数据收集的质量和速度， 将使我们的旧（过时）仪器无法进行的观测成为可能。

 DESCRIPTION (provided by applicant): We request funds for the purchase of a Leica EM UC7 ultramicrotome for sectioning biological and biomaterials samples at room temperatures. The new instrument will help provide for the ultramicrotomy needs of over 60 user laboratories at the University of Massachusetts Medical School (UMMS) and the Graduate School of Biomedical Sciences (GSBS). The new ultramicrotome will be housed in the Core Electron Microscopy Facility of the Medical School, and will replace a 30-year old Reichert-Jung Ultracut E, that while still functioning, is now considered obsolete, has become unreliable, and is no longer supported by the manufacturer (parts or service). Ultramicrotomes are used to cut thin sections of cells and tissues that are required for understanding their detailed structure and the changes in structure that underlie human disease. Many tissue-engineering strategies involve the delivery of cells via porous polymer scaffolds. Obtaining sections of the emerging tissue is necessary to analyze the characteristics of the microscopic environment. The Core Electron Microscopy facility supports projects ranging from studies of the structure of muscle, microtubule formation and stabilization, stem cell and germ cell differentiation, cilia and flagell structure, just to mention a few. All are important topics in the understanding of the normal function and structure of the human body and all require ultramicrotomy. The Leica EM UC7 Ultra-microtome offers a range of outstanding features and benefits over our old Reichert-Jung Ultracut E model. Each user, from absolute beginner students to our most skilled ultra- microtomist will benefit from this modern instrument. The eucentric movement of the viewing system allows examination of sections even with lowered water level (as with Lowycryl, and LR White resins, used in immune-labeling projects). The touch sensitive control unit of the Leica EM UC7 enables fast and safe alignment of knife and specimen with help files and prompts, simplifying the trimming- process while preparing samples for sectioning. Funding this grant will help us provide state-of-the-art instrumentation for our shared research Electron Microscopy Core Facility, utilized by over 100 individual researchers and their graduate students as well as the faculty and graduate students at several colleges in the surrounding area. The new instrument will greatly enhance the ability of our investigators and students to carry out their microtomy-related studies, it will greatly improve the quality and speed of data collection, and it will make possible types of observations that are impossible with our older (obsolete) instrument.