Ultramicrotome

超薄切片机

基本信息

批准号：
9075291
负责人：
Gregory Hendricks
金额：
$ 5.32万
依托单位：
UNIV OF MASSACHUSETTS MED SCH WORCESTER
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-03-15 至 2017-03-14
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): We request funds for the purchase of a Leica EM UC7 ultramicrotome for sectioning biological and biomaterials samples at room temperatures. The new instrument will help provide for the ultramicrotomy needs of over 60 user laboratories at the University of Massachusetts Medical School (UMMS) and the Graduate School of Biomedical Sciences (GSBS). The new ultramicrotome will be housed in the Core Electron Microscopy Facility of the Medical School, and will replace a 30-year old Reichert-Jung Ultracut E, that while still functioning, is now considered obsolete, has become unreliable, and is no longer supported by the manufacturer (parts or service). Ultramicrotomes are used to cut thin sections of cells and tissues that are required for understanding their detailed structure and the changes in structure that underlie human disease. Many tissue-engineering strategies involve the delivery of cells via porous polymer scaffolds. Obtaining sections of the emerging tissue is necessary to analyze the characteristics of the microscopic environment. The Core Electron Microscopy facility supports projects ranging from studies of the structure of muscle, microtubule formation and stabilization, stem cell and germ cell differentiation, cilia and flagell structure, just to mention a few. All are important topics in the understanding of the normal function and structure of the human body and all require ultramicrotomy. The Leica EM UC7 Ultra-microtome offers a range of outstanding features and benefits over our old Reichert-Jung Ultracut E model. Each user, from absolute beginner students to our most skilled ultra- microtomist will benefit from this modern instrument. The eucentric movement of the viewing system allows examination of sections even with lowered water level (as with Lowycryl, and LR White resins, used in immune-labeling projects). The touch sensitive control unit of the Leica EM UC7 enables fast and safe alignment of knife and specimen with help files and prompts, simplifying the trimming- process while preparing samples for sectioning. Funding this grant will help us provide state-of-the-art instrumentation for our shared research Electron Microscopy Core Facility, utilized by over 100 individual researchers and their graduate students as well as the faculty and graduate students at several colleges in the surrounding area. The new instrument will greatly enhance the ability of our investigators and students to carry out their microtomy-related studies, it will greatly improve the quality and speed of data collection, and it will make possible types of observations that are impossible with our older (obsolete) instrument.

描述（由适用提供）：我们要求购买LiCA EM UC7超级机体的资金，用于在室温下对生物学和生物材料样品进行切除。新工具将有助于满足马萨诸塞大学医学院（UMMS）和生物医学科学研究生院（GSBS）的60多种用户实验室的超微切开术需求。新的超大核组将安置在医学院的核心电子显微镜设施中，并将取代30岁的Reichert-Jung Ultracut E，尽管现在仍被认为是过时的，但它已经过时了，已经变得不可靠，并且不再由制造商（零件或服务）支持。超级机体用于切割细胞和时机的薄部分，这些细胞和时机是理解其详细结构以及构成人类疾病的结构变化所必需的。许多组织工程策略涉及通过多孔聚合物支架传递细胞。对于分析微观环境的特征，必须获得新兴组织的切片。核心电子显微镜设施支持项目，从肌肉结构，微管形成和稳定，干细胞和生殖细胞分化，纤毛和鞭毛结构，仅提及一些。所有这些都是对人体正常功能和结构的理解的重要主题，所有这些都需要超细切开术。 Leica EM UC7 Ultra-Microtome比我们旧的Reichert-Jung Ultracut E模型提供了一系列出色的功能和好处。从绝对的初学者到我们最熟练的超微型主义者，每个用户都将从这种现代乐器中受益。即使在免疫标记项目中使用的水位降低（如降低水平和LR白树脂），观看系统的欧盟运动也允许检查切片（如LowyCryl和LR White树脂）。 Leica EM UC7的触摸敏感控制单元可以通过帮助文件和提示快速，安全地对齐标本，并在准备示例进行分割时简化修剪过程。资助这笔赠款将有助于我们为我们的共享研究电子显微镜核心设施提供最先进的仪器，该工具由100多名个人研究人员及其研究生以及周围几所学院的教职员工和研究生使用。新工具将大大提高我们的研究人员和学生进行与微切开术相关的研究的能力，它将大大提高数据收集的质量和速度，并且它将将使我们较旧（过时的）仪器不可能进行观察的可能类型。