Ultramicrotome

 描述(由申请人提供)：我们申请资金购买徕卡EM UC7超微切割机，用于在室温下对生物和生物材料样品进行切片。新仪器将帮助满足马萨诸塞大学医学院(UMMS)和生物医学研究生院(GSBS)60多个用户实验室的超显微手术需求。新的超微切割机将安装在医学院的核心电子显微镜设备中，并将取代已有30年历史的Reichert-Jung Ultrut E，后者虽然仍在运行，但现在被认为是过时的，已变得不可靠，不再受到制造商(部件或服务)的支持。超微显微镜被用来切割细胞和组织的薄片，这些薄片是了解细胞和组织的详细结构以及人类疾病背后的结构变化所必需的。许多组织工程策略涉及通过多孔聚合物支架输送细胞。获取新生组织的切片对于分析微观环境的特征是必要的。核心电子显微镜设备支持的项目包括肌肉结构、微管形成和稳定、干细胞和生殖细胞分化、纤毛和鞭毛结构等，仅举几例。所有这些都是了解人体正常功能和结构的重要课题，都需要超显微手术。徕卡EM UC7超微型切割机与我们的老款Reichert-Jung Ultraut E型号相比，具有一系列突出的功能和优势。每个用户，从绝对的初学者到我们最熟练的超显微切割师，都将从这台现代仪器中受益。观察系统的向心运动使得即使在水位较低的情况下也可以检查切片(如免疫标记项目中使用的Lowycryl和LR White树脂)。徕卡EM UC7的触敏控制单元可以通过帮助文件和提示快速、安全地对准刀具和标本，从而简化修剪过程，同时为切片准备样品。资助这笔资金将帮助我们为我们共享的研究电子显微镜核心设备提供最先进的仪器，超过100名个人研究人员和他们的研究生以及周围地区几所大学的教职员工和研究生使用该设备。新仪器将极大地增强我们的研究人员和学生进行显微手术相关研究的能力，它将极大地提高数据收集的质量和速度，并且它 将使我们的旧(过时)仪器不可能进行的观测类型成为可能。

 DESCRIPTION (provided by applicant): We request funds for the purchase of a Leica EM UC7 ultramicrotome for sectioning biological and biomaterials samples at room temperatures. The new instrument will help provide for the ultramicrotomy needs of over 60 user laboratories at the University of Massachusetts Medical School (UMMS) and the Graduate School of Biomedical Sciences (GSBS). The new ultramicrotome will be housed in the Core Electron Microscopy Facility of the Medical School, and will replace a 30-year old Reichert-Jung Ultracut E, that while still functioning, is now considered obsolete, has become unreliable, and is no longer supported by the manufacturer (parts or service). Ultramicrotomes are used to cut thin sections of cells and tissues that are required for understanding their detailed structure and the changes in structure that underlie human disease. Many tissue-engineering strategies involve the delivery of cells via porous polymer scaffolds. Obtaining sections of the emerging tissue is necessary to analyze the characteristics of the microscopic environment. The Core Electron Microscopy facility supports projects ranging from studies of the structure of muscle, microtubule formation and stabilization, stem cell and germ cell differentiation, cilia and flagell structure, just to mention a few. All are important topics in the understanding of the normal function and structure of the human body and all require ultramicrotomy. The Leica EM UC7 Ultra-microtome offers a range of outstanding features and benefits over our old Reichert-Jung Ultracut E model. Each user, from absolute beginner students to our most skilled ultra- microtomist will benefit from this modern instrument. The eucentric movement of the viewing system allows examination of sections even with lowered water level (as with Lowycryl, and LR White resins, used in immune-labeling projects). The touch sensitive control unit of the Leica EM UC7 enables fast and safe alignment of knife and specimen with help files and prompts, simplifying the trimming- process while preparing samples for sectioning. Funding this grant will help us provide state-of-the-art instrumentation for our shared research Electron Microscopy Core Facility, utilized by over 100 individual researchers and their graduate students as well as the faculty and graduate students at several colleges in the surrounding area. The new instrument will greatly enhance the ability of our investigators and students to carry out their microtomy-related studies, it will greatly improve the quality and speed of data collection, and it will make possible types of observations that are impossible with our older (obsolete) instrument.