Polymicrobial interactions in a biofilm of periodontopathic bacteria

牙周病细菌生物膜中的多种微生物相互作用

• 批准号: nhmrc : 400327
• 负责人: Dr Nada Slakeski
• 金额: $ 31.64万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2006
• 资助国家: 澳大利亚
• 起止时间: 2006-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/polymicrobial-interactions-biofilm-periodontopathic-bacteria/76259
• 关键词: Polymicrobial; interactions; biofilm; periodontopathic; bacteria

Chronic periodontitis is a bacterial-associated inflammatory disease of the supporting tissues of the teeth, which results in the destruction of tooth support and ultimately leads to tooth loss. The disease is a major public health problem with a large economic burden and has been associated with an increased risk of cardiovascular disease and pre-term birth and low birth weight. Three bacterial species in a biofilm (dental plaque) have been closely associated with chronic periodontitis in humans and a fourth bacterial species has been suggested to assist colonisation of the biofilm by the three pathogenic species. The aim of this project is to use DNA microarray and proteomic techniques to study the interaction of the four bacterial species grown together in a polymicrobial biofilm. In particular genes of two of the pathogens, Porphyromonas gingivalis and Treponema denticola, important for biofilm formation and virulence in an animal model will be identified. This information will provide insight into the molecular processes of dental plaque formation and therfore molecular targets for the development of specific inhibitors that may have utility in the treatment and prevention of chronic periodontitis.

慢性牙周炎是一种与细菌相关的牙齿支持组织炎症性疾病，导致牙齿支持结构破坏，最终导致牙齿脱落。该疾病是一个重大的公共卫生问题，具有巨大的经济负担，并与心血管疾病和早产和低出生体重的风险增加有关。生物膜（牙菌斑）中的三种细菌物种与人类慢性牙周炎密切相关，并且已经提出第四种细菌物种有助于三种致病物种在生物膜中定殖。本项目的目的是使用DNA微阵列和蛋白质组学技术来研究在多微生物生物膜中一起生长的四种细菌的相互作用。具体地，将鉴定对动物模型中的生物膜形成和毒力重要的两种病原体牙龈卟啉单胞菌和齿垢密螺旋体的基因。这些信息将提供深入了解牙菌斑形成的分子过程，并因此为开发可能在治疗和预防慢性牙周炎中具有实用性的特异性抑制剂提供分子靶点。