Iron/hemin transport systems of Porphyromonas gingivalis

牙龈卟啉单胞菌的铁/血红素转运系统

• 批准号: nhmrc : 145831
• 负责人: Dr Nada Slakeski
• 金额: $ 26.14万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2001
• 资助国家: 澳大利亚
• 起止时间: 2001-01-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/ironhemin-transport-systems-porphyromonas-gingivalis/97940
• 关键词: Iron; hemin; transport; systems; Porphyromonas

Periodontitis is a bacterial-associated disease of the supporting structures of the teeth and can result in tooth loss. The disease is classified as a major public health problem with an enormous economic burden. A bacterium, Porphyromonas gingivalis, has now been implicated as a major causative agent of periodontitis in adults. To survive and grow within humans pathogenic bacteria must be able to acquire essential elements, including iron. The human host has developed a variety of mechanisms to restrict the amount of iron available as a means of limiting the growth of bacterial pathogens. In response to this iron limitation Porphyromonas gingivalis has developed novel ways of obtaining this essential element. We have identified a novel iron transport system that P. gingivalis uses to obtain iron from the human host. A component of this transport system, FetB is a surface protein antigen. The aims of this project are to: i. Determine the mechanism by which this novel iron transport system functions by genetic manipulation of the organism using molecular biology techniques. ii. Determine how important this transport system is for the pathogenicity of the bacterium. iii. Prepare the FetB protein using recombinant DNA technology. iv. Test the recombinant FetB protein and another iron transport protein (Tlr) in animal models to determine their efficacy as a vaccine for periodontitis. The expected outcomes of this research are:- i. the understanding of how P. gingivalis transports iron. ii. an understanding of the importance of iron transport systems to the pathogenicity of the bacterium. iii. a P. gingivalis vaccine with efficacy in animals based on an iron transport protein(s).

牙周炎是一种与细菌有关的牙齿支撑结构疾病，可导致牙齿脱落。该疾病被列为具有巨大经济负担的重大公共卫生问题。一种细菌，牙龈卟啉单胞菌，现在被认为是成人牙周炎的主要病原体。为了在人体内生存和生长，致病菌必须能够获得必需的元素，包括铁。人类宿主已经发展出多种机制来限制铁的数量，作为限制细菌病原体生长的一种手段。为了应对这种铁的限制，牙龈卟啉单胞菌开发了获得这种必需元素的新方法。我们已经确定了一种新的铁运输系统，牙龈卟啉卟啉菌利用它从人类宿主获得铁。作为这个运输系统的一个组成部分，FetB是一种表面蛋白抗原。该项目的目的是：i.利用分子生物学技术，通过对生物体进行遗传操作，确定这种新型铁转运系统的功能机制。2。确定这种运输系统对细菌致病性的重要性。3。利用重组DNA技术制备FetB蛋白。iv.在动物模型中测试重组FetB蛋白和另一种铁转运蛋白（Tlr），以确定它们作为牙周炎疫苗的功效。本研究的预期结果是：1 .了解牙龈卟啉卟啉转运铁的方式。2。了解铁转运系统对细菌致病性的重要性。3。一种基于铁转运蛋白对动物有效的牙龈假单胞菌疫苗。