High-resolution visual imaging for morphometric and histological analysis of squamate morphology

本提案中要求的设备是为了支持我提交的NSERC发现资助提案“鳞片的宏观进化：整合发育，生理和历史数据”。我正在开发一种形态成像设备，作为我作为多伦多大学密西沙加分校生物系新CTLA助理教授的研究的一部分。该设备的核心组件是这里描述的显微镜系统。该系统由奥林巴斯szx16立体显微镜组成，配有Qimaging MicroPublisher 5.0 RTV CCD彩色摄像机。该系统将用于生成近期和化石鳞片爬行动物骨骼的形态计量学分析数据，以及薄切片骨骼的组织学分析数据，这些研究将用于重建脊椎动物发育、生理和环境变化之间的历史关系。在多伦多大学密西沙加分校，我找不到相应的资源。该系统将被安置在一个提供的研究空间中，并将成为学生培训的一个组成部分，特别是在解剖学，形态计量学，组织学和发展的主题，以及显微镜的实际应用，图像生成和处理，数据库建设和结果发表。

The equipment requested in this proposal is to support my submitted NSERC Discovery Grant proposal "Macroevolution of squamates: Integrating developmental, physiological, and historical data".  I am developing a morphological imaging facility as a component of my research as a new CTLA Assistant Professorship in the Department of Biology, University of Toronto at Mississauga.  The core component of this facility is the microscopy system described here.  The system consists of an Olympus SZX 16 stereomicroscope housing a Qimaging MicroPublisher 5.0 RTV CCD color camera.  The system will be used to generate data for morphometric analysis of recent and fossil squamate reptile osteology and for histological analysis of thin-sectioned bone, studies that will be used to reconstruct the historical relationship between development, physiology, and environmental change in vertebrates.  There are no equivalent resources available to me at the University of Toronto at Mississauga.  The system will be housed in a provided research space, and will be an integral part of student training, specifically in the topics of anatomy, morphometrics, histology, and development, as well as the practical applications of microscopy, image generation and processing, database construction, and publication of results.