Multiphon imaging for understanding social brain function in tadpoles

多声子成像用于了解蝌蚪的社交脑功能

• 批准号: 10717610
• 负责人: NA Ji
• 金额: $ 63.65万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-15 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10717610
• 关键词: 3-Dimensional; Ablation; Address; Afferent Neurons; Amphibia; Animals; Area; Atlases; Behavior; Brain; Brain imaging; Caregivers; Cells; Code; Communication; Communities; Complex; Cues; Dancing; Dangerousness; Data; Decision Making; Development; Fire - disasters; Fluorescence; Fluorescence Microscopy; Foundations; Future; Generations; Goals; Hunger; Image; Imaging Device; Imaging Techniques; Individual; Infant; Label; Larva; Learning; Life; Longitudinal Studies; Mammals; Measurement; Measures; Methods; Microscopy; Modeling; Mothers; Motor; Nature; Neurons; Neurophysiology - biologic function; Neurosciences; Norepinephrine; Nutritional; Odors; Olfactory Learning; Olfactory Pathways; Optics; Organism; Parent-Child Relations; Parents; Pattern; Performance; Photons; Pigmentation physiologic function; Poison; Protocols documentation; Proxy; Rana; Research; Resolution; Siblings; Signal Transduction; Slice; Smell Perception; Social Behavior; Social Environment; Social Interaction; Specificity; Statistical Data Interpretation; Stimulus; Tadpoles; Techniques; Technology; Testing; Time; Visual; Visualization; Xenopus; adaptive optics; comparative; cranium; experimental study; feeding; image reconstruction; imaging approach; imaging capabilities; imprint; improved; in vivo; in vivo imaging; model organism; multi-photon; multiphoton imaging; multiphoton microscopy; neonate; neural; neural circuit; neuroethology; neuroimaging; olfactory bulb; olfactory sensory neurons; pup; response; social; social attachment; tool; two-photon

Project Summary Mother-infant bonding is a key relationship that lays a foundation for wellness throughout life. Social recognition is an important component of this relationship, as infants imprint on the smell of their mothers and use olfaction to distinguish their mother from others. This behavior is widespread in mammals, although the complexity of the mammalian brain and paucity of tools and repeated imaging capabilities to study neonate pups make it difficult to obtain a precise mechanistic understanding of the basic brain mechanisms for mother-infant bonding and communication in young. Mother-infant recognition and bonding also occur in other taxa, suggesting there are other species in which to study generalizable neural principles of parent-offspring interactions. Recently our labs have developed (1) poison frog tadpoles as a model to study social brain development and (2) multiphoton imaging approaches to enable in vivo recording of pigmented aquatic larvae. We propose to develop multiphoton in vivo imaging approaches to study the encoding of maternal odors in a social poison frog tadpole that can distinguish their mother from strangers. We hypothesize that the olfactory response landscape changes throughout development as tadpoles learn the smell of their mothers. We predict that a tadpole’s ability to distinguish their mother from strangers coincides with an increase in olfactory cells that fire with specificity to maternal odors, measured by multiphoton imaging of olfactory system neural activity repeatedly across development. Prior to these experiments, we will evaluate the performance of various multiphoton imaging techniques for optically accessible depth inside the brain of multiple species of poison frog tadpoles with varying levels of pigmentation. We will validate the results with rigorous statistical analyses and comparison of neural activity data with immunohistological imaging of brain slices and activity-dependent sequencing of olfactory sensory neurons. Understanding how amphibians learn and encode individual conspecific identity will either reveal alternative mechanisms of encoding olfactory-based recognition or which patterns of olfactory encoding are ancestral or generalizable features of vertebrate olfactory processing. Importantly, our approach will result in the development of multiphoton approaches for whole brain imaging of pigmented aquatic animals, which is a valuable toolkit of broad use for the neuroscience community. Successful completion of this project will allow us to obtain proof-of-principle data for proposing in vivo imaging of tadpole brains throughout development, in a comparative context, and within the framework of ethologically relevant behaviors, which is crucial for future R01 applications. Furthermore, establishing a recording protocol in tadpoles will allow for other aspects of neural function in amphibians, a research area that has thus far been limited due to technological constraints. In summary, our proposed research will help elucidate core olfactory imprinting principles in a relatively simple tadpole brain and reveal how these principles for olfactory encoding may generalize across vertebrate taxa.

项目摘要 母婴结合是一种关键的关系，为一生的健康奠定了基础。社会 识别是这种关系的一个重要组成部分，因为婴儿会对母亲的气味留下印记， 用嗅觉来区分它们的母亲。这种行为在哺乳动物中很普遍，尽管 哺乳动物大脑的复杂性以及研究新生儿的工具和重复成像能力的缺乏 幼崽使得很难获得对基本大脑机制的精确机械理解， 幼儿时期的母婴联系和沟通。母婴识别和结合也发生在其他 分类群，这表明还有其他物种，其中研究一般的神经原则的父母-子女 交互.最近，我们的实验室开发了（1）毒蛙蝌蚪作为研究社会脑的模型 发展和（2）多光子成像方法，使在体内记录色素水生幼虫。 我们建议开发多光子体内成像方法来研究母体气味的编码， 一种群居毒蛙蝌蚪，它能区分自己的母亲和陌生人。我们假设嗅觉 在整个发育过程中，反应景观会随着蝌蚪学习母亲的气味而变化。我们预测 蝌蚪区分母亲和陌生人的能力与嗅觉细胞的增加是一致的 通过嗅觉系统神经活动的多光子成像测量， 在发展过程中不断重复。在这些实验之前，我们将评估各种 多光子成像技术用于多种毒蛙脑内的光学可达深度 有着不同程度色素的蝌蚪。我们将通过严格的统计分析来验证结果， 神经活动数据与脑切片免疫组织学成像和活动依赖性 嗅觉感觉神经元的测序。了解两栖动物如何学习和编码个体 同种身份要么揭示编码嗅觉识别的替代机制， 嗅觉编码的模式是脊椎动物嗅觉处理的祖先或可概括的特征。 重要的是，我们的方法将导致全脑成像的多光子方法的发展， 色素水生动物，这是一个有价值的工具包，广泛用于神经科学界。 该项目的成功完成将使我们能够获得用于提出体内成像的原理证明数据 在比较的背景下，在行为学的框架内， 相关行为，这对未来R 01应用至关重要。此外，建立记录协议 蝌蚪的研究将允许两栖动物神经功能的其他方面，这一研究领域迄今为止一直是 由于技术限制而受到限制。总之，我们的研究将有助于阐明核心嗅觉 在一个相对简单的蝌蚪大脑中的印记原则，并揭示这些原则是如何为嗅觉编码 可以在脊椎动物分类群中推广。