Diode array UV- visible spectrophotometer

二极管阵列紫外可见分光光度计

• 批准号: 345636-2007
• 负责人: Yang, Jian
• 金额: $ 2.18万
• 依托单位: University of Saskatchewan
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326052
• 关键词: Diode; array; UV; visible; spectrophotometer

The current proposal is for the purchase of a diode array UV-visible spectrophotometer to support the pharmaceutical, biological and environmental research programs in the College of Pharmacy and Nutrition at the University of Saskatchewan.  This piece of equipment will be installed in the Core Equipment Facility, a new multi-user equipment room which is centrally located in the College and houses equipment which is available to all faculty, postdoctoral fellows, graduate students, technicians and summer students in the College of Pharmacy and Nutrition. This facility employs an experienced lab manager to maintain the equipment and train all users in the proper care and use of the equipment. A diode-array UV-visible spectrophotometer provides instantaneous measurement of the UV-visible spectrum and aids in characterizing individual drugs and/or metabolites.  Compared to a conventional UV-visible spectrophotometer, it offers fast scanning, excellent sensitivity, superior wavelength reproducibility, as well as  exceptional ruggedness and reliability.  An important usage of diode-array instruments is in the kinetic studies of enzymes.  The instrument requested is especially crucial for our current kinetic studies of the catalytic enzymes involved in the biodegradation of pentachlorophenol, a major environmental pollutant in North America, because pentachlorophenol and it's catalytic products are difficult to measure by a single frequency and some of the catalytic products are not stable enough for the traditional high-performance liquid chromatography studies. Furthermore, the data obtained using the diode array UV Visible spectrophotometer can be extrapolated for use on conventional HPLC-UV(single wavelength)detectors such that maximum sensitivity can be achieved using simpler technology.

目前的提议是购买一个二极管阵列紫外可见分光光度计，以支持萨斯喀彻温大学药学与营养学院的药物、生物和环境研究项目。该设备将安装在核心设备设施中，这是一个新的多用户设备室，位于学院的中央，容纳设备，可供药学与营养学院的所有教职员工、博士后研究员、研究生、技术人员和暑期学生使用。该设施聘请了一名经验丰富的实验室经理来维护设备，并对所有用户进行适当的设备维护和使用培训。二极管阵列紫外-可见分光光度计提供了紫外-可见光谱的瞬时测量，并有助于表征单个药物和/或代谢物。与传统的紫外-可见分光光度计相比，它具有扫描速度快、灵敏度高、波长重现性好、以及特殊的坚固性和可靠性。二极管阵列仪器的一个重要用途是在酶的动力学研究中。所需的仪器对于我们目前对五氯酚生物降解的催化酶动力学研究尤其关键。五氯酚是北美主要的环境污染物。由于五氯苯酚及其催化产物很难用单一频率测定，而且一些催化产物不够稳定，不适合传统的高效液相色谱研究。此外，使用二极管阵列紫外可见分光光度计获得的数据可以外推用于传统的高效液相-紫外(单波长)检测器，从而可以使用更简单的技术获得最大的灵敏度。