Real-time PCR instrument

近年来发展起来的研究基因表达的最强大的工具之一是实时聚合酶链式反应(PCR)。这项技术可以精确量化微量的核酸(DNA或RNA)。所要求的实时聚合酶链式反应仪器将用于舍布鲁克大学五名生物学教授的实验室，使他们能够从事高水平的研究计划，并以重要的方式为这一尖端技术方面的高素质人员培训做出贡献。该设备将使以下项目成为可能：1)主要组织相容性复合体(MHC)II类基因的调节，这对免疫系统的功能至关重要；2)植物细胞程序性死亡机制的研究，这是植物正常发育和抵御病原体所必需的；3)双重亮氨酸拉链激酶(DLK)家族对基因调控的影响，它参与控制细胞分化和程序性细胞死亡的信号级联；4)植物先天不团结的分子机制研究，这对植物抵御病原体的能力至关重要；5)研究染色质对核糖体基因的调节作用，核糖体基因是产生蛋白质合成的细胞机器(核糖体)所必需的。所要求的设备将对我们在具有国际竞争力的水平上开展高质量研究和培训高素质人员的能力作出决定性贡献。

One of the most powerful tools to study gene expression developed in recent years is that of real-time polymerase chain reaction (PCR). This technology allows a precise quantification of minute amounts of nucleic acids (DNA or RNA). The requested real-time PCR instrument will be used in the laboratories of five biology professors of the Université de Sherbrooke and allow them to pursue high-level research programs and contribute in an important manner to the training of highly qualified personnel in this state-of-the-art technology. The projects made possible by this equipment will include the following studies: 1) the regulation of the major histocompatibility complex (MHC) class II locus, which is of crucial importance for the function of the immune system; 2) the study of programmed cell death mechanisms in plants, which are essential for normal plant development and defense against pathogens; 3) the effects on gene regulation of the dual leucine-zipper kinase (DLK) family of protein kinases, which are involved in the signaling cascades controlling cellular differentiation and programmed cell death; 4) the study of the molecular mechanisms of plant innate imunity, which are essential for the capacity of plants to defend themselves against pathogens; and 5) the study of the role of chromatin for the regulation of ribosomal genes, which are essential to generate the cellular machines (ribosomes) for the synthesis of proteins. The requested equipment will contribute in a decisive manner to our ability to carry out high quality research at an internationally competitive level and to train highly qualified personnel.