Bioplex system for multiplexing microassays of biomarkers

Bioplex系统使用流式细胞术测量复杂生物样品中的生物标志物：蛋白质分析（细胞因子、趋化因子、生长因子、激素、细胞信号蛋白、转录因子和核受体）、基因表达谱和基因型分析。可同时对50 uL样品进行多达100次选择分析，以最小的样品使用实现高通量。对少量生物液体进行多次同时检测的主要优势是：a)减少动物使用和成本，b)对研究结果进行更综合的检查，c)刺激新的探索性合作和跨学科研究，d)提高研究吞吐量和生产力，e)降低实验室研究成本f)减少将样品发送到其他实验室的需要。与其他设备选项相比，Bioplex将在广泛的浓度范围内提供多功能性，准确，敏感的分析。它将对nserc资助的研究生产力、创新和HQP培训产生直接影响。研究项目：营养物质和营养状况在不同发育和代谢状态中的作用是重要和活跃的研究领域。我们使用各种感染、感染方案和炎症应激源来探索不同发育状态动物在不同程度营养应激下的细胞病理和免疫反应。研究考察了对细胞信号通路、激素、载脂蛋白、蛋白质磷酸化状态、细胞因子和趋化因子、生长因子、蛋白激酶、转录因子、核受体、基因表达谱的影响。我们对少量生物液体（血清/血浆、羊水、组织提取物、细胞和胚胎培养物、牛奶样本）进行检测，以调查营养、感染和免疫之间的相互关系。对HQP研究训练的影响：学院和研究所120名研究生中的许多人将受益于增加他们研究的机械性质，通过获得整合许多成果的经验，以及对代谢的综合性质和多学科研究的好处的赞赏。

The Bioplex System uses flow cytometry to measure biomarkers in complex biological samples: protein analysis (cytokines, chemokines, growth factors, hormones, cell signalling proteins, transcription factors and nuclear receptors), gene expression profiling and genotype analysis. Up to 100 selected assays can be conducted simultaneously on 50 uL samples, allowing high throughput with minimal sample use. Key advantage of performing multiple simultaneous assays on small quantities of biological fluids are to: a) Decrease animal use and costs, b) Provide a more integrated examination of research findings, c) Stimulate new exploratory collaborations and interdisciplinary research, d) Increase research throughput and productivity, e) Decrease costs of laboratory research f) Decrease our need to send samples out to other labs. In comparison to other equipment options, the Bioplex will provide versatility, and accurate, sensitive analyses over a wide range of concentrations. It will have an immediate impact on NSERC-funded research productivity, innovation and HQP training.  Research Programs: The roles of nutrients and nutritional status in different developmental and metabolic states are important and active research areas. We use a variety of infections, infection protocols, and inflammatory stressors to explore cellular pathologies and immune responses under varying degrees of nutritional stress in animals in different developmental states. Research examines effects on cell signalling pathways, hormones, apolipoproteins, phosphorylation state of proteins, cytokines and chemokines, growth factors, protein kinases, transcription factors, nuclear receptors, gene expression profiles. We perform assays in small quantities of biological fluids (serum/plasma, amniotic fluid, and extracts of tissues, cell and embryo cultures, milk samples) to investigate the interrelationships among nutrition, infection and immunity. Impact on Research Training of HQP:  Many of the 120 graduate students in the School and Institute would benefit by increasing the mechanistic nature of their research, and by gaining experience integrating many outcomes, and an appreciation of the integrative nature of metabolism and the benefits of multidisciplinary research.