A visualized patch clamp recording system

我需要一个可视化的膜片钳记录系统来扩展我目前对中枢听觉系统的研究计划(NSERC发现资助)。该设备将使我们能够对听觉中脑的细胞生理学进行研究。与盲录相比，可视化膜片钳记录方法具有允许详细研究离子通道和提高信号处理基础上的生理事件的分辨率的优点。可视化膜片钳记录方法对于研究发育中的神经元，如幼年动物的听觉脑干中的神经元尤为必要。这些神经元太小或太脆弱，无法使用尖锐的电极记录或盲膜片钳方法进行穿透。使用可视化的膜片钳记录系统可以实现稳定的记录。然后，可以应用各种药物来研究听觉神经元的特定突触受体和离子通道，并可以应用荧光染料来检查被记录的神经元的形态和位置。我请求拨款购买必要的设备，包括显微镜、显微操作器和渗透压计，用于可视化膜片钳记录系统。利用这个系统，我们将在细胞和分子水平上研究听觉神经元的信息处理、可塑性、发育和功能障碍的神经机制。本实验室开发的可视化膜片钳记录系统将对听觉领域具有重要的价值，为未来的研究提供新的方向，确保本实验室保持在听神经科学的前沿。这项设备赠款将使听觉神经生理学的进步成为可能，这将对加拿大的神经科学大有裨益。由于我的团队中的几名研究生需要可视化的膜片钳记录系统，以确保为拟议的研究提供高质量的记录和可靠的结果，因此拨款申请是紧迫的。

A visualized patch clamp recording system is needed to extend my current program of research on the central auditory system (NSERC Discovery Grant supported). The equipment will allow us to carry out the investigation of cellular physiology of the auditory midbrain. The visualized patch clamp recording method has the advantage over blind recordings of allowing detailed investigation of ion channels and improved resolution of physiological events underlying signal processing. The visualized patch clamp recording method is especially necessary for studying developing neurons, such as those in the auditory brainstem of young animals. These neurons are too small or fragile to impale using a sharp electrode recording or a blind patch clamp method. Stable recordings will be achieved with a visualized patch clamp recording system. It is then possible to apply various pharmacological agents to investigate specific synaptic receptors and ion channels of auditory neurons, and fluorescent dyes to examine the morphology and location of the neuron being recorded.   I am requesting the grant money to purchase essential equipment, including a microscope, a micromanipulator and an osmometer, for a visualized patch clamp recording system. With this system we will study neuronal mechanisms of information processing, plasticity, development, and dysfunction of auditory neurons at cellular and molecular levels. Development of a visualized patch clamp recording system in my laboratory would be greatly valuable to the auditory field, providing a novel direction for future research and ensuring that our laboratory remains on the cutting edge of auditory neuroscience. The advances in auditory neurophysiology that will be made possible by this equipment grant will be of great benefit to Canadian neuroscience. The grant request is urgent as several graduate students in my group require a visualized patch clamp recording system to ensure high quality recordings and reliable results for the proposed research.