Characterizing the functional heterogeneity of the mouse paralaminar nucleus

表征小鼠板旁核的功能异质性

• 批准号: 10678525
• 负责人: David Saxon
• 金额: $ 3.5万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10678525
• 关键词: Action Potentials; Adolescence; Adolescent; Adult; Age; Amygdaloid structure; Animal Model; Area; Atlases; Behavior; Behavioral; Biological Models; Brain; Brain region; Categories; Cell Count; Cell Nucleus; Cells; Classification; Closure by clamp; Collaborations; Data; Databases; Development; Electrophysiology (science); Emotional; Fellowship; Goals; Growth; Heterogeneity; Hippocampus; Human; Individual; Injections; Knowledge; Label; Lateral; Light; Link; Maps; Measures; Membrane; Mental disorders; Microscopy; Molecular; Monkeys; Morphology; Mus; N-Methylaspartate; Neurons; Output; Pathology; Pattern; Phenotype; Physiological; Physiology; Play; Population; Post-Traumatic Stress Disorders; Pregnancy; Primates; Process; Property; Research; Role; Slice; Social Behavior; Source; Spike Potential; Subgroup; Synapses; Techniques; Testing; Training; Universities; Vertebral column; Viral; Virus; Work; autism spectrum disorder; base; biocytin; confocal imaging; density; electrical property; emotional behavior; entorhinal cortex; excitatory neuron; experimental study; gamma-Aminobutyric Acid; immunoreactivity; intercalation; mouse model; neuronal cell body; neuropsychiatric disorder; optogenetics; patch clamp; reconstruction; response; social

PROJECT SUMMARY/ABSTRACT The development of the amygdala includes an increase in size and cell number during adolescence, which is thought to confer maturation of emotional and social behaviors before adulthood. Perturbations in this process are associated with neuropsychiatric disorders and behavioral pathology; yet the cellular components of adolescent amygdala development are unknown. In humans, the paralaminar nucleus of the amygdala (PL) contains a large population of immature excitatory neurons that delay their maturation until adolescence. The PL may be a major contributor to adolescent amygdala development, but the physiology and connectivity of the region is yet to be investigated. Our recent work has shown the existence of an orthologous population in the mouse amygdala, opening a tractable model system to study the PL in detail. My initial electrophysiology experiments indicate putative physiological subgroups within the adult mouse PL. Based on past primate studies and the Allen Mouse Brain Connectivity atlas, inputs to and outputs from the adult PL may also show diversity. The goal of this proposal is to define the functional identity of the adult PL by classifying intrinsic electrical and morphological neuronal subtypes, dissecting their synaptic input patterns, and mapping their outputs. The training proposed in the advanced techniques of viral tracing, light sheet microscopy, whole-cell patch clamp, optogenetics, and morphological reconstruction will facilitate the exploration into the functional phenotypes of mouse PL neurons. These proposed studies are crucial to understanding the role of the PL in amygdala function and behavior, as well possible involvement in neuropsychiatric disorders.

项目总结/文摘