Service contracts for transmission electron microscopes in NSERC Guelph regional integrated imaging facility (GRIIF)

NSERC圭尔夫区域综合成像设施（GRIIF），前身为NSERC圭尔夫区域STEM设施（GRSF），为加拿大研究界提供各种调查功能。多年来，它已经建立了一个专业知识和显微镜仪器基地，使其成为世界上独一无二的。GRIIF包含透射电子显微镜（TEM），原子力显微镜（AFM），全息显微镜，共聚焦显微镜及其操作所需的所有辅助设备。最近，GRIIF已经成为少数几个能够进行cryoTEM的设施之一，cryoTEM是少数几种可以在其自然水合状态下以高分辨率可视化生物物质的方法之一。GRIIF在阐明原核结构及其物理特性方面建立了世界声誉，特别强调微生物如何与其周围环境相互作用。它还帮助建立了一个新的地球科学领域，称为地球微生物学。GRIIF擅长于各种各样的研究课题，如可视化传染病的病原体、抗生素的靶点、对环境重要的微生物（例如，极端微生物、金属呼吸细菌、生物修复剂、古细菌等），和微生物生物膜（在疾病、环境和工业中活跃）。GRIIF一直是加拿大细菌疾病网络-国家卓越中心（CBDN-NCE）的主要显微镜设施，目前是先进食品和材料网络-国家卓越中心（AFMnet-NCE）的主要设施。通常情况下，每年约有75-100名来自加拿大和国外的研究人员（高级研究人员，博士后学生和研究生）得到GRIIF的帮助。

The NSERC Guelph Regional Integrated Imaging Facility (GRIIF), which was formerly the NSERC Guelph Regional STEM Facility (GRSF), serves a variety of investigative functions for the Canadian research community. Over the years it has built up an expertise and microscope instrumentation base that makes it one-of-a-kind worldwide. GRIIF contains transmission electron microscopes (TEMs), atomic force microscopes (AFMs), a holographic microscope, confocal microscopes and all necessary ancillary equipment for their operation. Recently, GRIIF has endeavored to become one of the few facilities capable of cryoTEM, which is one of the few ways bio-matter can be visualized at high resolution in its natural hydrated state. GRIIF has established a worldwide reputation in the elucidation of prokaryotic structure and its physical properties with special emphasis on how microorganisms interact with their surroundings. It has also helped establish a new earth science field named geomicrobiology. GRIIF excels in such diverse research topics as visualizing the agents of infectious disease, the targets of antibiotics, environmentally important microbes (e.g., extremophiles, metal-respiring bacteria, bioremediation agents, archaea, etc.), and microbial biofilms (that are active in disease, the environment and industry). GRIIF has been the primary microscope facility for the Canadian Bacterial Disease Network - National Centre of Excellence (CBDN-NCE) and is currently the primary facility for the Advanced Food and Materials Network - National Centre of Excellence (AFMnet-NCE). Typically ~75-100 researchers (senior investigators, post-doctoral students and graduate students) from Canada and abroad are aided with their research by GRIIF each year.