Service contracts for transmission electron microscopes in NSERC Guelph regional integrated imaging facility (GRIIF)

NSERC Guelph地区综合成像设施(GRIIF)的前身是NSERC Guelph地区STEM设施(GRSF)，为加拿大研究界提供各种调查职能。多年来，它已经建立了一个专业技术和显微镜仪器基地，使其成为全球独一无二的。GRIIF包括透射式电子显微镜(TEM)、原子力显微镜(AFM)、全息显微镜、共聚焦显微镜以及运行所需的所有辅助设备。最近，GRIIF努力成为少数几个能够进行低温透射电子显微镜的设施之一，这是在自然水合状态下以高分辨率可视化生物物质的为数不多的方法之一。GRIIF在阐明原核生物结构及其物理性质方面建立了世界声誉，特别强调微生物如何与环境相互作用。它还帮助建立了一个新的地球科学领域，名为地球微生物学。GRIIF在可视化传染病病原体、抗生素的目标、对环境有重要影响的微生物(如极端细菌、金属呼吸细菌、生物修复试剂、古生菌等)和微生物生物膜(在疾病、环境和工业中活跃)等多个研究课题上表现出色。GRIIF一直是加拿大细菌病网络-国家英才中心(CBDN-NCE)的主要显微镜设施，目前是先进食品和材料网络-国家英才中心(AFMnet-NCE)的主要设施。通常每年约有75-100名来自加拿大和国外的研究人员(高级研究员、博士后和研究生)得到GRIIF的资助。

The NSERC Guelph Regional Integrated Imaging Facility (GRIIF), which was formerly the NSERC Guelph Regional STEM Facility (GRSF), serves a variety of investigative functions for the Canadian research community. Over the years it has built up an expertise and microscope instrumentation base that makes it one-of-a-kind worldwide. GRIIF contains transmission electron microscopes (TEMs), atomic force microscopes (AFMs), a holographic microscope, confocal microscopes and all necessary ancillary equipment for their operation. Recently, GRIIF has endeavored to become one of the few facilities capable of cryoTEM, which is one of the few ways bio-matter can be visualized at high resolution in its natural hydrated state. GRIIF has established a worldwide reputation in the elucidation of prokaryotic structure and its physical properties with special emphasis on how microorganisms interact with their surroundings. It has also helped establish a new earth science field named geomicrobiology. GRIIF excels in such diverse research topics as visualizing the agents of infectious disease, the targets of antibiotics, environmentally important microbes (e.g., extremophiles, metal-respiring bacteria, bioremediation agents, archaea, etc.), and microbial biofilms (that are active in disease, the environment and industry). GRIIF has been the primary microscope facility for the Canadian Bacterial Disease Network - National Centre of Excellence (CBDN-NCE) and is currently the primary facility for the Advanced Food and Materials Network - National Centre of Excellence (AFMnet-NCE). Typically ~75-100 researchers (senior investigators, post-doctoral students and graduate students) from Canada and abroad are aided with their research by GRIIF each year.