Resources, Workforce Development, and Animal Models for the Rutgers RBL

总体摘要 罗格斯大学区域生物遏制实验室(RBL)作为中央设施执行 生物安全三级(BSL3)高威胁生物制剂的治疗、发病机制和诊断研究 重点关注结核分枝杆菌和SARS-CoV-2，以及其他A、B和C类病原体。 RBL为罗格斯大学、美国东北部和 在全国范围内，同时也通过合作和全球合作与公司和学术机构接触 研究合同。这项提议将提供支持，以增强RBL完成其研究和 在支持不断扩大的教职员工的同时，生物治疗中心还负责应对/预防大流行的任务。我们 建议通过改进RBL设施、支持服务、BSL3实践发展来实现这些目标 通过执行三个目标来提供和执行和提供特别服务：目标1.建立一个机制 管理、维护和运营(FMMO)核心。目标2.建立BSL-3实践核心(实践 核心)。目标3.建立生物遏制研究支持服务核心，致力于开发动物模型 BSL3病原体和相关支持服务(动物模型和相关服务，或AMRS核心)。这个 FMMO核心将为常规畜牧业提供BSL3和ABSL3服务、管理和监督， 支持研究人员的微生物学和病毒学服务资助研究项目，同时确保 有效运作和维护第三条干线设施，并提供训练有素的工作人员为第三条线提供支持 建筑系统和设备。业务核心将制定和维护标准操作程序 对RBL BSL3实验室的研究进行培训，包括最佳做法、应急反应、废物 管理，运输，畜牧业，选择代理商特定的做法和库存。它还将开发和进行 进行生物安全和灾害演习，并与RBL网络内的其他BSL3实验室以及地方、 州和联邦卫生机构协调行动并计划联合应对新的传染病 威胁。AMRS核心将开发关键的动物模型，包括SARS-CoV2和高致病性动物模型 流感病毒传播、新冠肺炎PASC、结核病后肺功能损害和药物治疗 模型，然后支持赠款资助的调查人员在这些模型的性能与高级 需要仪器来分析这些感染模型及其在BSL3环境中的组织/细胞。 这三个核心加在一起，将显著增强RBL的短期和长期能力，以解决 需要在BSL3实验室环境中研究的关键生物疗法和新出现的传染病，同时还 提高我们应对下一次突发公共卫生事件或大流行的能力。

Overall ABSTRACT The Rutgers University Regional Biocontainment Laboratory (RBL) serves as a central facility to perform biosafety level three (BSL3) therapeutic, pathogenesis, and diagnostic research on high threat biological agents with a focus on Mycobacterium tuberculosis and SARS-CoV-2, as well as other category A, B, and C pathogens. The RBL serves academic and commercial entities within Rutgers University, the Northeast United States and nationally, while also engaging globally with companies and academic institutions through collaborations and research contracts. This proposal will provide support that enhances the RBL’s ability to fulfill its research and biothreat response/pandemic preparedness missions while also supporting an expanding faculty/staff. We propose to accomplish these goals by improving the RBL facilities, support services, BSL3 practice development and implementation and special services offerings though the execution of three aims: Aim 1. Establish a Facility management, maintenance and operations (FMMO) core. Aim 2. Establish a BSL-3 Practices core (Practice core). Aim 3. Establish a biocontainment research support service core devoted to developing animal models of BSL3 pathogens and associated support services (Animal models and related services, or AMRS core). The FMMO core will provide BSL3 and ABSL3 services, management and oversight, for routine animal husbandry, microbiology and virology services in support of investigators grant funded research projects while ensuring efficient operations and maintenance of the BSL3 facilities and providing trained staff to support the BSL3 building systems and equipment. The Practice core will develop and maintain standard operating procedures and training for research in the RBL BSL3 laboratories including best practices, emergency response, waste management, shipping, husbandry, select agent-specific practices and inventory. It will also develop and conduct biosecurity and disaster drills and liaise with other BSL3 laboratories within the RBL network as well as local, state and federal health agencies to coordinate operations and plan for joint responses to new infectious disease threats. The AMRS core will develop critical animal models including those of SARS-CoV2 and highly pathogenic influenza virus transmission, COVID-19 PASC, pulmonary impairment after TB (PIAT), and drug treatment models, and then support grant funded investigators in the performance of these models along with the advanced instrumentation needed to analyze these infected models and their tissues/cells in a BSL3 environment. Together, these three cores will significantly enhance the near- and long-term abilities of the RBL to address critical biothreats and emerging infectious diseases requiring study in a BSL3 laboratory setting, while also increasing our capacity to respond to the next public health emergency or pandemic.