Characterization of HLS5, a novel tumor suppressor gene

新型抑癌基因 HLS5 的表征

• 批准号: nhmrc : 212070
• 负责人: Prof Peter Klinken
• 金额: $ 27.14万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2002
• 资助国家: 澳大利亚
• 起止时间: 2002-01-01 至 2004-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/characterization-hls5-novel-suppressor-gene/94569
• 关键词: Characterization; HLS5; novel; tumor; suppressor

HLS5 is a novel gene that we recently discovered in our laboratory. Preliminary investigations suggest that HLS5 is similar to a family of genes which act as DNA regulators. We have shown that HLS5 is found on a region of chromosome 8 which is often deleted in human cancers, suggesting that HLS5 is a new tumour suppressor gene i.e.. damage to this gene may be responsible for the formation of certain types of cancer (specifically breast and prostate). Other evidence to support the claim that HLS5 is a tumour suppressor gene comes from the proteins it associates with these partner molecules are involved in DNA repair or DNA regulation. When we introduced HLS5 into cancer cells, it slowed their growth and reduced their ability to form tumours. The aim of this project therefore, is to undertake a detailed analyses of the HLS5 gene and to determine the function of its protein product. A combination of approaches will be used in this study. We will: (i) alter the amount of HLS5 expression in cancer cells, (ii) characterize the proteins which bind to HLS5, (iii) determining where HLS5 localizes in the cell, (iv) analyze mice with lack the gene for HLS5, (v) assess the involvement of HLS5 in a human leukemia (vi) analyze HLS5 messenger RNA which produces the protein, and (vii) determining the structure of HLS5 protein. These studies should provide valuable information on how HLS5 functions, as well as its role in cancer formation.

HLS5 是我们实验室最近发现的一个新基因。初步研究表明，HLS5 与充当 DNA 调节因子的基因家族相似。我们已经证明，HLS5 存在于 8 号染色体的一个区域，该区域在人类癌症中经常被删除，这表明 HLS5 是一种新的肿瘤抑制基因，即该基因的损伤可能与某些类型的癌症（特别是乳腺癌和前列腺癌）的形成有关。支持 HLS5 是肿瘤抑制基因这一说法的其他证据来自于它与参与 DNA 修复或 DNA 调节的伙伴分子相关的蛋白质。当我们将 HLS5 引入癌细胞时，它减缓了癌细胞的生长并降低了它们形成肿瘤的能力。因此，该项目的目的是对 HLS5 基因进行详细分析并确定其蛋白质产物的功能。本研究将结合使用多种方法。我们将：(i) 改变癌细胞中 HLS5 的表达量，(ii) 表征与 HLS5 结合的蛋白质，(iii) 确定 HLS5 在细胞中的定位，(iv) 分析缺乏 HLS5 基因的小鼠，(v) 评估 HLS5 在人类白血病中的参与情况，(vi) 分析产生该蛋白质的 HLS5 信使 RNA，以及 (vii)确定HLS5蛋白的结构。这些研究应该提供有关 HLS5 如何发挥作用及其在癌症形成中的作用的有价值的信息。