CHARACTERIZATION OF THE NOVEL LEUKEMIA-INDUCING GENE MLF1

新型白血病诱导基因 MLF1 的特征

• 批准号: nhmrc : 254668
• 负责人: Prof Peter Klinken
• 金额: $ 26.26万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-01-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/characterization-novel-leukemia-gene-mlf1/100940
• 关键词: CHARACTERIZATION; NOVEL; LEUKEMIA; INDUCING; GENE

All of the circulating blood cells (including red cells and white cells) arise from haemopoietic stem cells found in the adult bone marrow. Stem cells gradually develop into one cell type. Once they have started down a particular pathway, they no longer generate cells of another pathway (e.g. once a stem cell begins to develop into red blood cells, they do not change into white cells). However, there are a few examples of mature cells that have changed pathways. We generated a red blood cell line, which has on occasions changed into macrophages (white blood cells) when grown under stressful conditions. Using these cells, we identified a gene, HLS7, which was involved in the change to macrophages. An American group has independently shown this gene can cause leukemia (blood cancer). We have shown HLS7 has dramatic effects on normal blood development and, together with its effect on leukemias, demonstrates its importance to the blood system. Through our studies on how HLS7 works, we have identified another gene, Madm, which may be an important regulator of HLS7. We plan to investigate the normal function of HLS7, how it interacts with Madm and how it causes leukemias. Analysis of these genes will further our knowledge in this field of blood cell development and cancer formation.

所有的循环血细胞（包括红细胞和白色细胞）都来自成人骨髓中的造血干细胞。干细胞逐渐发育成一种细胞类型。一旦它们开始沿着特定的途径，它们就不再产生另一种途径的细胞（例如，一旦干细胞开始发育成红细胞，它们就不会变成白色细胞）。然而，有一些成熟细胞已经改变了途径的例子。我们产生了一个红细胞系，当在压力条件下生长时，它有时会变成巨噬细胞（白色血细胞）。使用这些细胞，我们确定了一个基因，HLS7，它参与了巨噬细胞的变化。一个美国研究小组已经独立地证明这种基因可以导致白血病（血癌）。我们已经表明HLS7对正常血液发育具有显著影响，并且连同其对白血病的影响一起，证明了其对血液系统的重要性。通过我们对HLS7如何工作的研究，我们已经确定了另一个基因Madm，它可能是HLS7的重要调节因子。我们计划研究HLS7的正常功能，它如何与Madm相互作用以及它如何导致白血病。对这些基因的分析将进一步加深我们在血细胞发育和癌症形成这一领域的知识。