Characterization of mast cell basophil haematopoesis in patients with asthma and drug allergies.

哮喘和药物过敏患者肥大细胞嗜碱性粒细胞造血的特征。

• 批准号: nhmrc : 113840
• 负责人: Prof Steven Krilis
• 金额: $ 27.5万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2000
• 资助国家: 澳大利亚
• 起止时间: 2000-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/characterization-mast-cell-drug-allergies/82140
• 关键词: Characterization; mast; cell; basophil; haematopoesis

The major emphasis of this investigation is to analyse blood for specific cells thought to be implicated in allergic disease from patients undergoing allergic reactions to medications and in patients with asthma and other allergies. Certain cells in the circulation of these patients are thought to be involved in the clinical manifestation seen in patients with asthma and other allergies. These cells have certain characteristics and for the first time our laboratory has identified a particular cell in the circulation of patients with asthma which we believe may be important in the pathogenesis of this condition. We propose to examine these cells in patients with asthma, those experiencing allergic reactions to drugs and other allergies and determine their characteristics and their growth responses to a number of growth factors which have been demonstrated to affect the growth of cells known as mast cells in the test tube. Once we have characterised these cells in terms of what particular proteins and other products they contain we will then try to correlate the number and type of cell with the clinical allergic state of the patient. Blood will be obtained from patients undergoing acute asthma and those in remission to determine if there is any specific relationship of these cells with acute allergic reactions. We will be also looking at how these cells regulate other physiological systems in the body, in particular possible explanations why patients that suffer acute allergic reactions develop significant drops in blood pressure. We can identify these cells with a specific antibody that we have used in our preliminary studies. This antibody binds the surface of these cells, however the specific protein to which this antibody binds has not been identified. We propose to identify this protein by using molecular biology techniques. These studies will provide us with important insights on the nature of these cells and may lead to new therapies.

本研究的主要重点是分析对药物过敏反应的患者以及哮喘和其他过敏患者的血液中被认为与过敏性疾病有关的特定细胞。这些患者血液循环中的某些细胞被认为与哮喘和其他过敏症患者的临床表现有关。这些细胞具有一定的特征，我们的实验室首次在哮喘患者的循环中发现了一种特殊的细胞，我们认为这可能是哮喘发病机制的重要因素。我们建议检查哮喘患者、药物过敏患者和其他过敏患者的这些细胞，并确定它们的特征和它们对许多生长因子的生长反应，这些生长因子在试管中已被证明会影响肥大细胞的生长。一旦我们根据这些细胞所含的特定蛋白质和其他产物确定了这些细胞的特征，我们就会试图将细胞的数量和类型与患者的临床过敏状态联系起来。将从急性哮喘患者和缓解期患者身上采集血液，以确定这些细胞与急性过敏反应是否存在任何特定关系。我们还将研究这些细胞如何调节身体的其他生理系统，特别是可能解释为什么患有急性过敏反应的患者会出现显著的血压下降。我们可以用我们在初步研究中使用的一种特殊抗体来识别这些细胞。这种抗体结合这些细胞的表面，但是这种抗体结合的特异性蛋白质还没有被确定。我们建议利用分子生物学技术对该蛋白进行鉴定。这些研究将为我们提供有关这些细胞性质的重要见解，并可能导致新的治疗方法。