Characterization of mast cell basophil haematopoesis in patients with asthma and drug allergies.

这项调查的主要重点是分析血液中被认为与药物过敏反应患者以及哮喘和其他过敏症患者的过敏性疾病有关的特定细胞。这些患者循环中的某些细胞被认为与哮喘和其他过敏症患者的临床表现有关。这些细胞具有某些特征，我们的实验室首次在哮喘患者的循环中发现了一种特殊的细胞，我们认为这可能在这种疾病的发病机制中起着重要作用。我们建议检测哮喘患者、对药物和其他过敏反应的患者的这些细胞，并确定它们的特征及其对一些生长因子的生长反应，这些生长因子已被证明影响试管中称为肥大细胞的细胞的生长。一旦我们根据这些细胞所含的特定蛋白质和其他产物确定了它们的特征，我们就会尝试将细胞的数量和类型与患者的临床过敏状态联系起来。将从急性哮喘患者和缓解期患者身上采集血液，以确定这些细胞是否与急性过敏反应有任何特定的关系。我们还将研究这些细胞如何调节体内的其他生理系统，特别是可能的解释，为什么遭受急性过敏反应的患者会出现血压显著下降。我们可以用我们在初步研究中使用的一种特定抗体来鉴定这些细胞。这种抗体结合在这些细胞的表面，但这种抗体结合的特定蛋白尚未确定。我们建议使用分子生物学技术来鉴定这种蛋白质。这些研究将为我们提供关于这些细胞性质的重要见解，并可能导致新的治疗方法。

The major emphasis of this investigation is to analyse blood for specific cells thought to be implicated in allergic disease from patients undergoing allergic reactions to medications and in patients with asthma and other allergies. Certain cells in the circulation of these patients are thought to be involved in the clinical manifestation seen in patients with asthma and other allergies. These cells have certain characteristics and for the first time our laboratory has identified a particular cell in the circulation of patients with asthma which we believe may be important in the pathogenesis of this condition. We propose to examine these cells in patients with asthma, those experiencing allergic reactions to drugs and other allergies and determine their characteristics and their growth responses to a number of growth factors which have been demonstrated to affect the growth of cells known as mast cells in the test tube. Once we have characterised these cells in terms of what particular proteins and other products they contain we will then try to correlate the number and type of cell with the clinical allergic state of the patient. Blood will be obtained from patients undergoing acute asthma and those in remission to determine if there is any specific relationship of these cells with acute allergic reactions. We will be also looking at how these cells regulate other physiological systems in the body, in particular possible explanations why patients that suffer acute allergic reactions develop significant drops in blood pressure. We can identify these cells with a specific antibody that we have used in our preliminary studies. This antibody binds the surface of these cells, however the specific protein to which this antibody binds has not been identified. We propose to identify this protein by using molecular biology techniques. These studies will provide us with important insights on the nature of these cells and may lead to new therapies.