Microplate UV/VIS spectrophotometer

申请资金用于购买Molecular Devices SpectraMax 190微孔板紫外/可见分光光度计，以及必要的软件和计算机系统（用于数据采集和分析）和石英微孔板（用于紫外范围内的吸光度测定）。这是为了替换一个不可修复和过时的Molecular Devices SpectraMax 250平板阅读器，该阅读器自1998年购买以来一直大量使用，但今年早些时候已经失效。SpectraMax 250已于1998年停产，不再提供服务，其备件已在过去两年中无法获得。微孔板分光光度计是一个高度通用和必要的研究工具，我的实验室人员。他们每天使用它进行各种各样的生化程序，包括高通量活性分析和植物代谢关键酶的动力学研究。微孔板读卡器也被广泛用于进行终点分析，如蛋白质、核酸和磷酸盐测定。在过去的5年里，我们将几个强大的分子生物学和基因组工具整合到我们的研究中，这就需要在260纳米处对DNA/RNA进行分光光度定量。SpectraMax 190进行了重大升级，增加了其他功能，例如在宽紫外-可见范围（190-850 nm）内完全可调，以及PathCheck传感器，该传感器可以感知每口井中的液体深度，并将吸光度值标准化到常规的1厘米路径长度。光谱扫描特性使我们能够确定各种化合物和纯化酶的吸光度光谱。Molecular Devices的微孔板分析软件（Softmax Pro）提供全面的仪器控制和数据还原。我的研究小组（现有博士后1人，副研究员1人）。（4名研究生和1名本科生）由于我们的SpectraMax 250车牌阅读器无法正常工作，他们的工作受到了严重干扰。如果得到资助，SpectraMax 190分光光度计将是我正在进行的研究计划和HQP培训的巨大资产。

Funds are requested to purchase a Molecular Devices SpectraMax 190 Microplate UV/VIS Spectrophotometer, together with requisite software and computer system (needed for data acquisition & analysis), and a quartz microplate (needed for absorbance determinations in the UV range). This is required to replace to an unrepairable and obsolete Molecular Devices SpectraMax 250 plate reader that has been heavily used since its purchase in 1998, but became non-functional earlier this year. The SpectraMax 250 was discontinued in 1998, is no longer serviced, and its spare parts have not been available for the past 2 years. The microplate spectrophotometer is a highly versatile and essential research tool for my laboratory personnel. They use it on a daily basis for a wide variety of biochemical procedures, including high-throughput activity assays and kinetic studies of key enzymes of plant metabolism. The microplate reader has also been extensively used to perform end-point assays such as protein, nucleic acid, and phosphate determinations. Over the past 5 years we have integrated several powerful molecular biological and genomic tools into our research, which necessitates spectrophotometric quantification of DNA/RNA at 260 nm. The SpectraMax 190 has been significantly upgraded with additional features such as being fully tunable over a broad UV-VIS range (190-850 nm), as well as  a PathCheck Sensor which senses the depth of liquid in each well and normalizes absorbance values to a conventional 1 cm pathlength. The spectral scanning feature enables us to determine absorbance spectra for various compounds and purified enzymes. Molecular Devices's microplate analysis software (Softmax Pro) provides comprehensive instrument control and data reduction. My research group (currently 1 post-doc, 1 res. assoc., 4 graduate & 1 undergraduate student) have experienced significant disruptions to their work since our SpectraMax 250 plate reader became non-functional. If funded, the SpectraMax 190 Spectrophotometer would be a huge asset for my ongoing research program and HQP training.