Microplate UV/VIS spectrophotometer

需要资金来购买一台分子设备公司的spectraMax 190微板UV/Vis分光光度计，以及必要的软件和计算机系统(用于数据采集和分析)和一块石英微板(用于测定紫外光范围内的吸光度)。这是为了取代不可修复和过时的分子器件spectraMax 250平板阅读器，该阅读器自1998年购买以来一直被大量使用，但在今年早些时候无法使用。SpetraMax 250于1998年停产，不再维修，其备件在过去两年中一直没有供应。对于我的实验室人员来说，微板分光光度计是一种用途广泛、必不可少的研究工具。他们每天使用它进行各种各样的生化程序，包括高通量活性分析和植物新陈代谢关键酶的动力学研究。微板阅读器还被广泛用于进行终点分析，如蛋白质、核酸和磷酸盐的测定。在过去的5年里，我们将几种强大的分子生物学和基因组学工具整合到我们的研究中，这就需要在260 nm处用分光光度法定量测定DNA/RNA。SpectraMax 190已大幅升级，增加了其他功能，例如在广泛的UV-Vis范围(190-850 nm)内完全可调，以及一个路径检查传感器，该传感器可以检测每口井中液体的深度，并将吸光度值归一化到传统的1厘米路径长度。光谱扫描功能使我们能够确定各种化合物和纯化酶的吸收光谱。分子设备公司的微板分析软件(Softmax Pro)提供全面的仪器控制和数据还原。我的研究小组(目前1个博士后，1个资源。ASSOC.，4名研究生和1名本科生)自从我们的spectraMax 250平板阅读器出现故障以来，他们的工作经历了严重的中断。如果获得资金，spectraMax 190分光光度计将是我正在进行的研究计划和HQP培训的一笔巨大资产。

Funds are requested to purchase a Molecular Devices SpectraMax 190 Microplate UV/VIS Spectrophotometer, together with requisite software and computer system (needed for data acquisition & analysis), and a quartz microplate (needed for absorbance determinations in the UV range). This is required to replace to an unrepairable and obsolete Molecular Devices SpectraMax 250 plate reader that has been heavily used since its purchase in 1998, but became non-functional earlier this year. The SpectraMax 250 was discontinued in 1998, is no longer serviced, and its spare parts have not been available for the past 2 years. The microplate spectrophotometer is a highly versatile and essential research tool for my laboratory personnel. They use it on a daily basis for a wide variety of biochemical procedures, including high-throughput activity assays and kinetic studies of key enzymes of plant metabolism. The microplate reader has also been extensively used to perform end-point assays such as protein, nucleic acid, and phosphate determinations. Over the past 5 years we have integrated several powerful molecular biological and genomic tools into our research, which necessitates spectrophotometric quantification of DNA/RNA at 260 nm. The SpectraMax 190 has been significantly upgraded with additional features such as being fully tunable over a broad UV-VIS range (190-850 nm), as well as  a PathCheck Sensor which senses the depth of liquid in each well and normalizes absorbance values to a conventional 1 cm pathlength. The spectral scanning feature enables us to determine absorbance spectra for various compounds and purified enzymes. Molecular Devices's microplate analysis software (Softmax Pro) provides comprehensive instrument control and data reduction. My research group (currently 1 post-doc, 1 res. assoc., 4 graduate & 1 undergraduate student) have experienced significant disruptions to their work since our SpectraMax 250 plate reader became non-functional. If funded, the SpectraMax 190 Spectrophotometer would be a huge asset for my ongoing research program and HQP training.