Microplate UV/VIS spectrophotometer

请拨资金用于购买一台Molecular Devices SpectraMax 190微量板紫外/维斯分光光度计，以及必要的软件和计算机系统（数据采集和分析所需）和一个石英微量板（测定紫外范围内的吸光度所需）。这是需要更换一个无法修复和过时的分子设备SpectraMax 250板阅读器，自1998年购买以来一直在大量使用，但今年早些时候变得不起作用。SpectraMax 250于1998年停产，不再维修，其备件在过去2年中无法获得。微孔板分光光度计是我实验室人员的一个高度通用和必不可少的研究工具。他们每天使用它进行各种生化程序，包括高通量活性测定和植物代谢关键酶的动力学研究。酶标仪还广泛用于进行终点测定，如蛋白质、核酸和磷酸盐测定。在过去的5年中，我们已经将几种强大的分子生物学和基因组工具整合到我们的研究中，这需要在260 nm处对DNA/RNA进行分光光度定量。SpectraMax 190经过显著升级，增加了其他功能，例如在宽UV-VIS范围（190-850 nm）内完全可调，以及PathCheck传感器，该传感器可感测每个孔中液体的深度，并将吸光度值标准化为传统的1 cm光程。光谱扫描功能使我们能够确定各种化合物和纯化酶的吸收光谱。Molecular Devices的微孔板分析软件（Softmax Pro）提供全面的仪器控制和数据简化。我的研究小组（目前有1名博士后，1名副研究员，自从我们的SpectraMax 250酶标仪无法正常工作以来，4名研究生和1名本科生的工作受到了严重干扰。如果获得资助，SpectraMax 190分光光度计将成为我正在进行的研究计划和HQP培训的巨大资产。

Funds are requested to purchase a Molecular Devices SpectraMax 190 Microplate UV/VIS Spectrophotometer, together with requisite software and computer system (needed for data acquisition & analysis), and a quartz microplate (needed for absorbance determinations in the UV range). This is required to replace to an unrepairable and obsolete Molecular Devices SpectraMax 250 plate reader that has been heavily used since its purchase in 1998, but became non-functional earlier this year. The SpectraMax 250 was discontinued in 1998, is no longer serviced, and its spare parts have not been available for the past 2 years. The microplate spectrophotometer is a highly versatile and essential research tool for my laboratory personnel. They use it on a daily basis for a wide variety of biochemical procedures, including high-throughput activity assays and kinetic studies of key enzymes of plant metabolism. The microplate reader has also been extensively used to perform end-point assays such as protein, nucleic acid, and phosphate determinations. Over the past 5 years we have integrated several powerful molecular biological and genomic tools into our research, which necessitates spectrophotometric quantification of DNA/RNA at 260 nm. The SpectraMax 190 has been significantly upgraded with additional features such as being fully tunable over a broad UV-VIS range (190-850 nm), as well as  a PathCheck Sensor which senses the depth of liquid in each well and normalizes absorbance values to a conventional 1 cm pathlength. The spectral scanning feature enables us to determine absorbance spectra for various compounds and purified enzymes. Molecular Devices's microplate analysis software (Softmax Pro) provides comprehensive instrument control and data reduction. My research group (currently 1 post-doc, 1 res. assoc., 4 graduate & 1 undergraduate student) have experienced significant disruptions to their work since our SpectraMax 250 plate reader became non-functional. If funded, the SpectraMax 190 Spectrophotometer would be a huge asset for my ongoing research program and HQP training.