Leica rotary microtome

徕卡旋转式切片机

• 批准号: 359199-2008
• 负责人: Yeung, Edward
• 金额: $ 1.48万
• 依托单位: University of Calgary
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=363003
• 关键词: Leica; rotary; microtome

Light microscopy is an essential tool for cell biology.  Our ability to make serial sections of specimens is a key technique in the study of an organism.  Improvements in the plastics used for specimen embedding  together with improvements in the microtome, such as the feature allowing for retraction during the return stroke, enables us to serially section plastic blocks.  These improvements greatly enhance the quality of histological studies.  This RTI grant application is to support my current Discovery Grant from NSERC.   The Reichert rotary microtome in my laboratory is approximately 25 years old. The manufacturer of this model, Reichert Jung is no longer in business.  Due to heavy use of this microtome, the drive shaft has already been replaced once 6 years ago.  Lately, the retraction of the cutting arm prior to specimen advancement has become erratic.  Serial sections cannot be obtained consistently. The inability to obtain serial sections makes interpretation difficult and also increases the time needed to section through a single block at 3 µm thickness.  This greatly reduces our productivity. Thus, in order to continue our structural studies, this application has to be made.From a teaching perspective, in recent years, fewer and fewer students are being trained in anatomical research.  This area of research is perceived to be old-fashioned.  On the contrary, rapid changes are taking place in different areas of microscopy and the need for assistance by microscopists continues to increase.  Through the use of different experimental systems and our experience, we continue to train high quality personnel in this area of research, but we need the tools in order to accomplish our goal.

光学显微镜是细胞生物学的重要工具。我们制作连续标本切片的能力是研究生物体的关键技术。用于包埋标本的塑料的改进以及切片机的改进，例如允许在回程时缩回的功能，使我们能够连续切片塑料块。这些改进大大提高了组织学研究的质量。这个RTI基金申请是为了支持我目前从NSERC获得的发现基金。 我实验室里的Reichert旋转切片机大约有25年的历史了。该型号的制造商Reichert Jung已不再营业。由于该切片机的大量使用，驱动轴已在6年前更换过一次。最近，在标本推进之前，切割臂的缩回变得不稳定。无法获得一致的连续切片。由于无法获得连续切片，因此难以进行解释，同时也增加了对3 µm厚的单个块体进行切片所需的时间。这大大降低了我们的生产率。因此，为了继续我们的结构研究，必须进行这种应用。从教学的角度来看，近年来，越来越少的学生接受解剖学研究的培训。这一领域的研究被认为是老式的。相反，显微镜的不同领域正在发生迅速的变化，对显微镜工作者的援助需求不断增加。通过使用不同的实验系统和我们的经验，我们继续在这一研究领域培养高素质的人才，但我们需要工具来实现我们的目标。