Multi-user digital imaging system

我们的五个研究小组要求获得一台计算机化的多用途成像仪，这将使我们能够从一系列分子生物学应用中捕获各种实验结果的数字化图像。成像仪由一个CCD摄像机组成，它被封装在一个灯箱上方的不透光室中，灯箱可以转换成白光或紫外光。在室内染色和适当光照后，可获得凝胶中溶解的蛋白质、DNA和RNA图像。相机的高分辨率和灵敏度与波长选择滤波器相结合，可以捕获和准确量化微弱和明亮的信号，并允许我们承担以前不可能使用旧成像仪的应用。五个小组正在进行的研究涵盖了一系列基础科学研究，包括：确定单个细胞膜跨越蛋白的结构，剖析酶的结构-功能关系，确定在细胞核中复制的寄生DNA元件如何借用宿主功能来确保其自身的维持，了解染色体DNA的包装如何影响我们基因的表达，比较不同脂肪酸对脂质代谢的影响及其对动脉粥样硬化的影响。该成像仪也将供本应用程序中指定的研究小组以外的研究小组使用，从而促进许多人的研究。

Our five research groups are requesting the acquisition of a computerized multi-purpose imager that will allow us to capture digitized images of a wide variety of experimental results from a range of molecular biology applications. The imager consists of a CCD camera enclosed in a light-tight chamber above a light box that can be converted to either white or ultraviolet light. Images of protein, DNA and RNA resolved in gels can be obtained after staining and appropriate illumination in the chamber. The high resolution and sensitivity of the camera combined with wavelength selective filters enable capture and accurate quantification of faint and bright signals and allowing us to undertake applications not previously possible with older imagers. The research being conducted by the five groups requesting this imager spans a range of basic science investigations including: determining the structure of individual cell membrane-spanning proteins, dissecting structure-function relationships of enzymes, determining how a parasitic DNA element replicating in a cell nucleus borrows host functions to ensure its own maintenance, understanding how packaging of our chromosomal DNA influences expression of our genes and comparing the effects of different fatty acids on lipid metabolism and their impact on atherosclerosis. The imager will also be accessible to research groups other than those named in this application thereby facilitating the research of many.