Supporting infrastructure required to install a caenorhabditis elegans functional genomic platform

细胞间多种信号的整合对于多细胞生物的发育至关重要。然而，这些机制仍然知之甚少。我们将使用微观蠕虫，秀丽隐杆线虫作为模型系统，以提供更好地了解胚胎发育过程中细胞信号整合事件中涉及的调控机制的组成和拓扑结构。要做到这一点，我们将开发一种整合基因组方法，包括生物信息互补层的生成和整合。需要一些设备来开发拟议的研究计划。这些设备是加拿大创新基金会为安装C. elegans功能基因组平台在UQAM科学学院。这些设备包括：（i）超低温冷冻器;（ii）热循环仪;（iii）冷冻台式离心机;（iv）冷培养器-振荡器;（v）微粒轰击系统。所需的设备是必不可少的不同的实验步骤的拟议计划的发展。它还需要完成一个C. elegans基因组平台将适合于未来大量项目和计划的开发。这些设备将使整个真核生物中保存的细胞信号整合机制的动态模型的产生，并参与了大量的人类病理学。因此，由拟议的基础设施支持的研究将更好地理解细胞信号事件。它还将为科学界提供动态模型，可用于更好地了解许多致病过程。这一基础设施还将为在我的实验室工作的学生提供支持高质量研究所需的设备，从而确保他们未来在学术界或工业界的职业生涯取得成功。

The integration by cells of multiple intercellular signals is of prime importance for the development of multicellular organisms. However, these mechanisms are still poorly understood. We will use the microscopic worm, Caenorhabditis elegans as a model system to provide a better understanding of the composition and topology of regulatory machineries involved in cell signaling integration events during embryonic development. To do so, we will develop an integrative genomic approach consisting in the generation and integration of complementary layers of biological information. Some pieces of equipment are required to develop the proposed research program. These equipments are required as a complement of the infrastructure requested to the Canadian Foundation for Innovation for the installation of a C. elegans functional genomic platform within the UQAM science faculty. These equipments consist in: (i) an ultra-low temperature freezer; (ii) a thermocycler; (iii) a refrigerated bench-top centrifuge; (iv) a cold incubator-shaker; (v) a microparticle bombardment system. The requested equipments are essential for the development of different experimental steps of the proposed program. It is also required to complete the installation of a C. elegans genomic platform that will be appropriate to the development of a large number of projects and program in the future. These equipments will enable the generation of dynamic models of cell signaling integration machineries conserved throughout Eukaryotes and involved in a large number of human pathologies. The research supported by the proposed infrastructure will consequently bring a better understanding of cell signaling events. It will also provide the scientific community with dynamic models that could be used to better understand number of pathogenic processes. This infrastructure will also provide the students working in my laboratory with equipment required to support a research of quality and consequently ensure the success of their future career in academia or in the industry.