Supporting infrastructure required to install a caenorhabditis elegans functional genomic platform

细胞对多种细胞间信号的整合对于多细胞生物体的发展至关重要。然而，人们对这些机制仍然知之甚少。我们将使用微小的线虫作为模型系统，以更好地了解胚胎发育过程中参与细胞信号整合事件的调控机制的组成和拓扑结构。为了做到这一点，我们将开发一种整合基因组方法，包括产生和整合互补的生物信息层。需要一些设备来开发拟议的研究计划。这些设备是对加拿大创新基金会要求的基础设施的补充，用于在UQAM科学学院内安装线虫功能基因组平台。这些设备包括：(1)超低温冷冻机；(2)热循环器；(3)冷藏台式离心机；(4)冷箱-摇床；(5)微粒轰击系统。所要求的设备对于开发拟议方案的不同实验步骤是必不可少的。还需要完成线虫基因组平台的安装，该平台将适合未来大量项目和计划的开发。这些设备将使细胞信号整合机制的动态模型的产生成为可能，这些机制在真核生物中保存，并参与大量人类病理。因此，在拟议基础设施的支持下进行的研究将使人们更好地了解细胞信号事件。它还将为科学界提供动态模型，可用于更好地了解致病过程的数量。这个基础设施还将为在我的实验室工作的学生提供所需的设备，以支持高质量的研究，从而确保他们未来在学术界或行业的职业生涯取得成功。

The integration by cells of multiple intercellular signals is of prime importance for the development of multicellular organisms. However, these mechanisms are still poorly understood. We will use the microscopic worm, Caenorhabditis elegans as a model system to provide a better understanding of the composition and topology of regulatory machineries involved in cell signaling integration events during embryonic development. To do so, we will develop an integrative genomic approach consisting in the generation and integration of complementary layers of biological information. Some pieces of equipment are required to develop the proposed research program. These equipments are required as a complement of the infrastructure requested to the Canadian Foundation for Innovation for the installation of a C. elegans functional genomic platform within the UQAM science faculty. These equipments consist in: (i) an ultra-low temperature freezer; (ii) a thermocycler; (iii) a refrigerated bench-top centrifuge; (iv) a cold incubator-shaker; (v) a microparticle bombardment system. The requested equipments are essential for the development of different experimental steps of the proposed program. It is also required to complete the installation of a C. elegans genomic platform that will be appropriate to the development of a large number of projects and program in the future. These equipments will enable the generation of dynamic models of cell signaling integration machineries conserved throughout Eukaryotes and involved in a large number of human pathologies. The research supported by the proposed infrastructure will consequently bring a better understanding of cell signaling events. It will also provide the scientific community with dynamic models that could be used to better understand number of pathogenic processes. This infrastructure will also provide the students working in my laboratory with equipment required to support a research of quality and consequently ensure the success of their future career in academia or in the industry.