Molecular characterization of the poplar-rust interaction

杨树-锈病相互作用的分子表征

• 批准号: 121366-2006
• 负责人: Séguin, Armand
• 金额: $ 1.41万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=366639
• 关键词: Molecular; characterization; poplar; rust; interaction

With their long life cycle, trees have developed distinct defense mechanisms allowing their long term survival. Forest pests can have adverse effects on tree growth and survival, yield and quality of wood, as well as wildlife habitat, resulting in either direct or indirect economic and environmental losses. In fact, the importance of pests and their negative impacts on forests (natural and planted) has been mainly understated. More recently, insect and disease movement has also been facilitated by the increased international trade of agricultural and forest products. Global climate change could also increase the occurrence and severity of pest outbreaks. The study of host-pathogen interactions in forest tree species has been limited primarily due to the lack of clearly defined biological systems. However, poplar-rust interactions have been relatively well documented. Poplar has become the model tree species for genomics research due to its extensive genetic resources, rapid early growth, ease of clonal propagation, and routine transformation protocols ( http://www.ornl.gov/sci/ipgc/). The sequence of its genome has also been recently completed. In addition, genomes of a cortege of associated microorganisms are being sequenced including the first tree pathogen, Melampsora poplar rust. During the last few years of this proposal we have developed a portfolio of genes induced during the defense response and have recently produced cDNA libraries of compatible and incompatible host-pathogen interactions in the poplar-rust pathosystem. We are planning to build upon this expertise to target functional and evolutionary analyses taking advantage of unique poplar-rust interaction systems. This will allow us to identify genes and expression profiles characteristic of these contrasting interactions and to identify genes that may be essential in host-pathogen recognition.

由于它们的生命周期长，树木已经发展出独特的防御机制，使它们能够长期生存。森林害虫可对树木生长和存活、木材产量和质量以及野生动物生境产生不利影响，造成直接或间接的经济和环境损失。事实上，虫害的重要性及其对森林（天然和人工）的负面影响基本上被低估了。最近，国际农业和森林产品贸易的增加也促进了昆虫和疾病的传播。全球气候变化也可能增加虫害爆发的发生率和严重程度。森林树种中寄主-病原体相互作用的研究一直受到限制，主要是由于缺乏明确定义的生物系统。然而，杨树锈病的相互作用已经得到了比较好的记录。白杨因其广泛的遗传资源、快速的早期生长、易于克隆繁殖和常规转化方案而成为基因组学研究的模式树种（http：//www.ornl.gov/sci/ipgc/）。其基因组序列最近也已完成。此外，一系列相关微生物的基因组正在测序中，包括第一个树木病原体，白杨锈病。在过去的几年中，我们已经开发了一个组合的防御反应过程中诱导的基因，最近产生的兼容和不兼容的主机-病原体相互作用的杨树锈病病理系统的cDNA文库。我们正计划利用这一专业知识，利用独特的杨树锈病相互作用系统进行功能和进化分析。这将使我们能够识别这些对比相互作用的基因和表达谱特征，并识别可能在宿主-病原体识别中必不可少的基因。