Molecular characterization of the poplar-rust interaction

杨树-锈病相互作用的分子表征

• 批准号: 121366-2006
• 负责人: Séguin, Armand
• 金额: $ 1.41万
• 依托单位: Université Laval
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=391127
• 关键词: Molecular; characterization; poplar; rust; interaction

With their long life cycle, trees have developed distinct defense mechanisms allowing their long term survival. Forest pests can have adverse effects on tree growth and survival, yield and quality of wood, as well as wildlife habitat, resulting in either direct or indirect economic and environmental losses. In fact, the importance of pests and their negative impacts on forests (natural and planted) has been mainly understated. More recently, insect and disease movement has also been facilitated by the increased international trade of agricultural and forest products. Global climate change could also increase the occurrence and severity of pest outbreaks. The study of host-pathogen interactions in forest tree species has been limited primarily due to the lack of clearly defined biological systems. However, poplar-rust interactions have been relatively well documented. Poplar has become the model tree species for genomics research due to its extensive genetic resources, rapid early growth, ease of clonal propagation, and routine transformation protocols ( http://www.ornl.gov/sci/ipgc/). The sequence of its genome has also been recently completed. In addition, genomes of a cortege of associated microorganisms are being sequenced including the first tree pathogen, Melampsora poplar rust. During the last few years of this proposal we have developed a portfolio of genes induced during the defense response and have recently produced cDNA libraries of compatible and incompatible host-pathogen interactions in the poplar-rust pathosystem. We are planning to build upon this expertise to target functional and evolutionary analyses taking advantage of unique poplar-rust interaction systems. This will allow us to identify genes and expression profiles characteristic of these contrasting interactions and to identify genes that may be essential in host-pathogen recognition.

由于其漫长的生命周期，树木已经形成了独特的防御机制，使它们能够长期生存。森林病虫害可对树木生长和存活、木材产量和质量以及野生动物栖息地产生不利影响，造成直接或间接的经济和环境损失。事实上，害虫及其对森林的负面影响(天然的和人工的)的重要性一直被低估。最近，农业和森林产品国际贸易的增加也为病虫害的传播提供了便利。全球气候变化也可能增加虫害暴发的发生和严重程度。林木寄主-病原菌相互作用的研究一直受到限制，这主要是由于缺乏明确定义的生物系统。然而，杨树与锈菌的相互作用已有相对较好的文献记载。杨树以其广泛的遗传资源、快速的早期生长、易于克隆繁殖和常规转化协议(http://www.ornl.gov/sci/ipgc/).)而成为基因组学研究的模式树种它的基因组序列最近也已经完成。此外，一系列相关微生物的基因组正在进行测序，其中包括第一种树木病原体--黑杨锈菌。在这一建议的最后几年中，我们开发了一系列在防御反应中诱导的基因组合，最近还建立了杨树-锈病病理系统中亲和性和不亲和性寄主-病原菌相互作用的cDNA文库。我们计划利用独特的杨树-锈菌相互作用系统，以这种专业知识为基础，针对功能和进化分析进行分析。这将使我们能够识别这些反差相互作用的基因和表达谱特征，并识别可能在宿主-病原体识别中至关重要的基因。