Studies toward the understanding of the cucumber necrosis virus uncoating process

了解黄瓜坏死病毒脱壳过程的研究

• 批准号: 43840-2006
• 负责人: Rochon, DAnn
• 金额: $ 1.98万
• 依托单位: University of British Columbia
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=390532
• 关键词: Studies; toward; understanding; cucumber; necrosis

Plant viruses cause disease in a variety of crops worldwide resulting in decreased crop quality and quantity. In Canada, viruses are responsible for serious diseases in several crops, including potato, wheat, many greenhouse crops, grapes, stone fruits, and others. Current methods for virus disease control are often not sufficiently robust to eliminate virus disease and, moreover, viruses can often overcome existing control methods via mutation.  Thus, new virus disease control strategies are required to complement and strengthen current strategies. Plant viruses are absolutely dependent on plant host cells for their multiplication and spread, and moreover, viruses must interact with components of the cell in a very specific way. Recent research on virus/host interactions has identified some of the required host components, and in some cases, it has been shown that removal or alteration of these components can decrease, if not eliminate, the ability of the virus to multiply. Thus, increasing knowledge in this area of virus/host interactions should assist in the design of new strategies for future control of virus infection. Plant virus particles, which consist of a genome that is surrounded by a protective coat, must be able to release the genome from the coat in order to begin the infection process.  Despite the importance of virus uncoating in the establishment and maintenance of infection, little is known about how uncoating occurs, where in the cell it occurs, or what specific host components are required.  The aim of this proposal is increase our knowledge of the virus uncoating process.  It is anticipated that this knowledge will contribute to the design of new strategies for future virus disease management.

植物病毒在世界范围内引起多种作物的病害，导致作物质量和数量下降。在加拿大，病毒是几种作物严重病害的罪魁祸首，包括土豆、小麦、许多温室作物、葡萄、核果等。目前的病毒病控制方法往往不足以消除病毒病，而且病毒往往可以通过突变来克服现有的控制方法。因此，需要新的病毒病控制策略来补充和加强现有的策略。植物病毒的繁殖和传播完全依赖于植物寄主细胞，而且病毒必须以一种非常特殊的方式与细胞的组成部分相互作用。最近关于病毒/宿主相互作用的研究已经确定了一些必需的宿主成分，在某些情况下，已经表明，移除或改变这些成分可能会降低病毒的繁殖能力，如果不是消除的话。因此，在病毒/宿主相互作用这一领域增加知识应有助于设计未来控制病毒感染的新战略。植物病毒颗粒由被保护性外壳包围的基因组组成，必须能够从外壳释放基因组，才能开始感染过程。尽管病毒去涂层在感染的建立和维持中很重要，但人们对病毒去涂层是如何发生的，在细胞中发生在哪里，或者需要哪些特定的宿主成分知之甚少。这项建议的目的是增加我们对病毒去涂层过程的了解。预计这种知识将有助于为未来的病毒疾病管理设计新的策略。