Molecular mechanisms of fungal mycoparasitism

真菌真菌寄生的分子机制

• 批准号: 137135-2008
• 负责人: JabajiHare, Suha
• 金额: $ 2.71万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=391374
• 关键词: Molecular; mechanisms; fungal; mycoparasitism

The fungus Stachybotrys elegans parasitizes the plant pathogen Rhizoctonia solani, the causal agent of Rhizoctonia disease of potato. Its success as a mycoparasite is due in large part to its possession of a suite of enzymes that degrade the cell walls of R. solani. These enzymes are used as anti-fungal compounds, and their effectiveness is enhanced when they are used in combination. The aim of this proposal is to contribute to the wider picture of mycoparasitism by providing a panoramic view of many biological processes occurring during microbe-microbe interaction. This will be accomplished by: (i) generating subtracted EST libraries comprising anonymous cDNA clones; (ii) identifying differentially expressed genes in both the mycoparasite and the host during their interaction using microarray analysis; (iii) cloning genes that are the most up-regulated genes and (iv) studying the differential and temporal expression of these genes during confrontation of S. elegans with hyphae and sclerotia of R. solani using quantitative real-time reverse-transcription (QRT)-PCR.

植物病原菌立枯丝核菌(Rhizoctonia Solani)是引起马铃薯丝核病的病原菌。它作为真菌寄生虫的成功在很大程度上归功于它拥有一套能降解立枯丝核菌细胞壁的酶。这些酶被用作抗真菌化合物，当它们联合使用时，它们的有效性会得到增强。这项提案的目的是通过提供微生物-微生物相互作用过程中发生的许多生物过程的全景图，有助于更广泛地了解真菌寄生现象。这将通过以下方式完成：(I)建立包含匿名cDNA克隆的消减EST文库；(Ii)利用微阵列分析找出真菌寄生虫和寄主在相互作用过程中的差异表达基因；(Iii)克隆上调表达最多的基因；(Iv)利用实时定量反转录(QRT)-PCR技术研究这些基因在立枯丝核菌与菌丝对峙过程中的差异和时间表达。