Quantitative PCR platform

定量PCR平台

• 批准号: 390289-2010
• 负责人: JabajiHare, Suha
• 金额: $ 3.12万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=443154
• 关键词: Quantitative; PCR; platform

The quantitative real-time PCR system measures amplifications of DNA and cDNA as it occurs cycle by cycle allowing for precise and accurate quantitative measurements during the exponential phase of PCR. The proposed instrument supports any real-time PCR application including SNP genotyping, gene expression profiling and gene detection. The proposed Mx30005 quantitative (q)PCR has the ability to detect and quantify multiple genes (maximum 5) simultaneously and validate the up-regulation of differentially expressed genes obtained from microarray analyses. Its registered software supports several analysis methods ranging from absolute to relative quantification, melting curve analysis and construction of standards. It automatically calculates relative quantities of gene expression, and create custom data reports that are easily interfaced with Microsoft, Excel and Powerpoint. It is user-friendly and the automation of this system allows researchers to maximize speed and convenience.

定量实时PCR系统测量DNA和cDNA的扩增，因为它一个循环一个循环地发生，从而允许在PCR的指数期期间进行精确和准确的定量测量。该仪器支持任何实时PCR应用，包括SNP基因分型、基因表达谱分析和基因检测。拟定的Mx30005定量（q）PCR能够同时检测和定量多个基因（最多5个），并验证从微阵列分析中获得的差异表达基因的上调。其注册软件支持多种分析方法，从绝对定量到相对定量、熔解曲线分析和标准品构建。它可以自动计算基因表达的相对数量，并创建自定义数据报告，可以轻松地与Microsoft，Excel和Powerpoint接口。它是用户友好的，该系统的自动化使研究人员能够最大限度地提高速度和便利性。