Mechanisms of S receptor kinase signalling in brassica self-incompatibility & related pathways in arabidopsis

通过信号转导对内外信号的感知是植物生长和生存的关键细胞过程。在植物中，受体激酶是最大的一类用于检测信号的受体，在植物的发育、生长和繁殖以及植物与微生物的相互作用和病原体防御系统中，受体激酶正在成为重要的调节因子。该资助的重点是研究s结构域受体激酶及其信号通路，并确定由这些信号蛋白调节的细胞事件。本研究计划的第一个方面是研究S受体激酶信号通路，阐明油菜（油菜、西兰花和卷心菜）自交不亲和花粉识别和排斥的分子机制。植物不能控制哪个花粉落在雌蕊上，但它们有一些机制，如自交不亲和，有利于非自交花粉受精，从而防止近亲繁殖。我的研究小组已经确定了一种新的调节这一过程的信号通路，在这项拨款申请中，我们提出了一系列的实验来继续研究这些信号蛋白如何导致自交不亲和花粉的排斥。本研究计划的第二个方面是研究这种独特的信号通路在调节植物生长和发育中的广泛程度。功能基因组学方法将用于定义模式植物拟南芥中相关信号基因家族的新生物学作用。此外，它们的细胞功能将被检查以揭示由这些蛋白质调节的假定信号通路和细胞事件。虽然这一领域正在取得令人兴奋的进展，但我们的整体知识仍然有限，特别是对受体激酶信号通路知之甚少。因此，本研究有望在理解植物受体激酶的功能及其在调节植物生长发育中的重要性方面取得新的重大进展。

The perception of internal and external signals through signal transduction is a critical cellular process for the growth and survival of plants.  In plants, receptor kinases comprise the largest group of receptors designed to detect signals, and are emerging as important regulators in plant development, growth, and reproduction as well as plant-microbe interactions and pathogen defence systems.  The focus of this grant is to study the S-Domain Receptor kinases and their signalling pathways, and to determine the cellular events regulated by these signalling proteins.  The first aspect of this research program is to study the S Receptor kinase signalling pathway and elucidate the molecular mechanisms involved in the recognition and rejection of self-incompatible pollen in Brassica (canola, broccoli, and cabbage).  Plants cannot control which pollen lands on the pistil, but they have mechanisms, such as self-incompatibility, that favour fertilization by non-self pollen, and thus, prevent inbreeding. My research group has identified a novel signalling pathway regulating this process, and in this grant application, a series of experiments are proposed to continue to study how these signalling proteins lead to rejection of self-incompatible pollen.  The second aspect of this research program is to examine how widespread this unique signalling pathway is in the regulation of plant growth and development.  Functional genomics approaches will be used to define novel biological roles for related signalling gene families in the model plant, Arabidopsis thaliana.  In addition, their cellular functions will be examined to unravel putative signalling pathways and cellular events regulated by these proteins. While exciting progress is emerging in this area, our overall knowledge is still limited, and in particular, little is known about receptor kinase signalling pathways.  Thus, this research is expected to generate novel and significant advancement into our understanding of how plant receptor kinases function and their importance in regulating plant growth and development.