A novel approach to determining true amino acid availability and requirements based on sulphur amino acid metabolism

猪肉产业是加拿大农业经济的主要贡献者，据估计每年的农场收入约为35亿美元。加拿大肉类加工业的年销售额接近150亿美元，其中约40%是猪肉。显然，一个成功的猪肉产业对加拿大整体经济至关重要。此外，加拿大50%的猪肉出口到全球90多个国家，因此我们的行业必须保持财政竞争力，生产高质量的产品并保持环境的可持续性。该项目的目的是开发一种创新和简单的方法来测定真硫氨基酸的需求和生物利用度。我们的方法是基于对硫氨基酸代谢的广泛接受的理解，即饮食提供的硫氨基酸和吸收的量超过生长所需的硫氨基酸将以游离硫酸盐或相关化合物的形式从尿液中排出。在本项目结束时，我们将提供一种新的测定含硫氨基酸真需要量和生物利用度的方法，并可能用于以尿硫酸盐为指示剂测定其他氨基酸真需要量和利用度的方法。这种方法背后的概念已经在人类和老鼠身上得到了很好的验证，因此成功的可能性非常大。测定真正氨基酸生物利用度的改进方法为制定最佳饲养计划提供了改进的平台，有可能使加拿大受益；反过来，这将有助于猪肉行业通过优化饲粮氨基酸含量，以尽可能低的成本获得最高质量的猪肉。避免氨基酸的过量配方也将通过减少氮的排泄来促进环境的可持续性，这是饮食配方不当时的常见结果。该提案将支持动物营养学领域的两名研究生和暑期学生的培训，该领域一直存在训练有素的人员供应不足的问题

The pork industry is a major contributor to the Canadian agriculture economy, with an estimated farm gate income of about $3.5 billion per year. The Canadian meat processing industry has annual sales of almost $15 billion, about 40% of which is pork. Clearly, a successful pork industry is important to the overall Canadian economy. In addition, 50% of Canada's pork is exported to more than 90 nations worldwide, so it is essential that our industry remains financially competitive, produces a high quality product and maintains environment sustainability. The objective of this project is to develop an innovative and simple method for the determination of true sulphur amino acid requirements and bioavailability. Our approach is based on the broadly accepted understanding of sulphur amino acid metabolism, namely that sulphur amino acids supplied by the diet and absorbed in amounts that exceed the requirement for growth will be excreted in the urine as fre sulphate or related compounds. At the end of this project, we will provide a new approach for the determination of true sulphur amino acid requirement and bioavailability, and a method that probably could be used to determine the true amino acid requirements and availability of other amino acids by using urinary sulphate as an indicator. The concepts behind this proposed method are well established in humans and rats, s the likelihood of success is very good. Improved methods for the determination of true amino acid bioavailability has the potential to benefit Canada by providing an improved platform for the formulation of optimum feeding programs; this, in turn, will assist the pork industry in achieving the highest quality pork at the lowest possible cost, through optimum dietary amino acid content. Avoiding over formulation of amino acids will also contribute to environmental sustainability by reducing nitrogen excretion, a common outcome when diets are improperly formulated. This proposal will support the training of two graduate students plus summer students in animal nutrition, a field that continually experiences an inadequate supply of trained personnel