In vitro characterization of human brain tumor "stem" cells: A lineage centric approach to phenotype characterization using multi-generation live cell imaging and tracking

人脑肿瘤“干”细胞的体外表征：使用多代活细胞成像和跟踪进行表型表征的以谱系为中心的方法

• 批准号: 351159-2008
• 负责人: Jervis, Eric
• 金额: $ 4.27万
• 依托单位: University of Waterloo
• 依托单位国家: 加拿大
• 项目类别: Collaborative Health Research Projects
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=402284
• 关键词: vitro; characterization; human; brain; tumor

The goal of this collaborative project is the development of novel and potentially high impact methods for diagnosis, prognosis and therapy planning in cancer - the end user of our research outcomes will be the clinical oncologist. We will achieve this goal by synergistically combining two recent conceptual advancements in medicine and engineering: the cancer stem cell hypothesis and the development of lineage informatics for in vitro clonal lineage tracking and analysis. Sophisticated robotic microscopes and informatics software developed in the laboratory of Jervis will be used to investigate the lineage tree structure and dynamic phenotype trajectories of human tumor stem cell lines and primary cells derived from tumor biopsy samples. The human cell lines have already received significant in vivo analysis in mouse models, so the tumor initiating properties of these cell lines in well defined. Correlation and pattern recognition analysis will be used to develop classification schemes for observed lineage trees and phenotypes with respect to in vivo mouse experiments and final disposition of the original tumor cell source patient.

该合作项目的目标是开发用于癌症诊断、预后和治疗规划的新颖且具有潜在高影响力的方法——我们研究成果的最终用户将是临床肿瘤学家。我们将通过协同结合医学和工程学领域的两个最新概念进展来实现这一目标：癌症干细胞假说和用于体外克隆谱系跟踪和分析的谱系信息学的发展。杰维斯实验室开发的精密机器人显微镜和信息学软件将用于研究人类肿瘤干细胞系和来自肿瘤活检样本的原代细胞的谱系树结构和动态表型轨迹。人类细胞系已经在小鼠模型中接受了重要的体内分析，因此这些细胞系的肿瘤启动特性已经明确。相关性和模式识别分析将用于针对体内小鼠实验和原始肿瘤细胞来源患者的最终处置观察到的谱系树和表型制定分类方案。