"Structure of hepadnaviral pre-genomic RNA".

“肝炎病毒前基因组RNA的结构”。

• 批准号: nhmrc : 104892
• 负责人: A/Pr Allison Jilbert
• 金额: $ 7.06万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2000
• 资助国家: 澳大利亚
• 起止时间: 2000-01-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/structure-hepadnaviral-pre-genomic-rna/76920
• 关键词: Structure; hepadnaviral; pre; genomic; RNA

Title: Structure of hepadnaviral pre-genomic RNA. We aim to study the replication strategy of human hepatitis B virus (HBV), a member of the hepadnavirus family. Hepadnaviruses infect hepatocytes in the liver and are released in high numbers into the bloodstream. Infection is transmitted by blood or sexual contact. Hepadnaviruses cause acute and chronic infection with varying degrees of liver disease. The HBV DNA genome is formed by copying of a viral pre-genome made of RNA, into DNA. This process is called reverse transcription and is performed by the viral polymerase. Reverse transcription occurs within viral nucleocapsids made of core antigen. After formation of the new viral DNA genome, nucleocapsids are enveloped in surface antigen and are released from the cell. It is assumed that 1 copy of HBV pre-genomic RNA is packaged within each viral nucleocapsid. However, members of the retrovirus family that have common evolutionary origins to hepadnaviruses and also replicate via reverse transcription, contain 2 copies of RNA. The human immunodeficiency virus (HIV), the AIDS virus, is a well-studied example. In HIV infection 2 RNA genomes are packaged into each nucleocapsid and form a dimeric RNA genome. The HIV RNA is able to fold into a series of stem loops that promote formation of dimers. During the reverse transcription step in HIV replication, the polymerase switches templates and forms new combined strains of virus. The project aims to determine if 2 copies of pre-genomic RNA are packaged into HBV nucleocapsids. HBV pre-genomic RNA is able to form stem loop structures similar to those in HIV and has the potential to form dimeric RNA. If 2 copies of HBV pre-genomic RNA are packaged this will allow us to redefine the viral replication strategy and to develop a greater understanding of the relationships between hepadnaviruses and retroviruses. The formation of dimers will also provide a mechanism for recombination between HBV strains.

标题：嗜肝DNA病毒前基因组RNA的结构。我们的目的是研究人类B肝炎病毒（HBV），嗜肝DNA病毒家族的成员的复制策略。嗜肝DNA病毒感染肝脏中的肝细胞并大量释放到血流中。感染通过血液或性接触传播。嗜肝DNA病毒引起急性和慢性感染，伴有不同程度的肝病。HBV DNA基因组是通过将由RNA制成的病毒前基因组复制成DNA而形成的。这个过程被称为逆转录，由病毒聚合酶完成。逆转录发生在由核心抗原组成的病毒核衣壳内。在新的病毒DNA基因组形成后，核衣壳被表面抗原包裹并从细胞中释放出来。假设1个拷贝的HBV前基因组RNA包装在每个病毒核衣壳内。然而，与嗜肝DNA病毒具有共同进化起源并且也通过逆转录复制的逆转录病毒家族的成员含有2个RNA拷贝。人类免疫缺陷病毒（HIV），即艾滋病病毒，就是一个经过充分研究的例子。在HIV感染中，2个RNA基因组被包装到每个核衣壳中并形成二聚体RNA基因组。HIV RNA能够折叠成一系列茎环，促进二聚体的形成。在HIV复制的逆转录步骤中，聚合酶转换模板并形成新的病毒组合株。该项目旨在确定是否有2个拷贝的前基因组RNA被包装到HBV核衣壳中。HBV前基因组RNA能够形成类似于HIV中的茎环结构，并且具有形成二聚体RNA的潜力。如果2个拷贝的HBV前基因组RNA被包装，这将使我们能够重新定义病毒复制策略，并对嗜肝DNA病毒和逆转录病毒之间的关系有更深入的了解。二聚体的形成也将为HBV毒株之间的重组提供机制。