High resolution respirometry system

所要求的设备是高分辨率呼吸测定系统，用于监测分离膜、线粒体和细胞在低氧水平下的有氧活动。由于技术原因，直到最近，大多数体外细胞和线粒体功能的测量都是在大大超过体内细胞水平的氧水平下进行的。接近体内氧气水平的测量在技术上具有挑战性，需要非常专业的设备，尽管全球只有一家制造商。这些部件由钛等材料制成，以最大限度地减少氧气回流。电子元件高度稳定，可在低氧水平下进行更可靠的测量。该系统包括用于在低氧水平下监测线粒体膜电位的滴定/电极系统。该设备将用于在低生理氧气水平下测量夏眠（休眠）和活动的肺鱼和蜗牛的分离线粒体中的广泛参数。需要这种设备来使我的研究计划向前推进，并验证我们对代谢性抑郁机制的假设。低氧水平对线粒体氧亲和力，膜电位和底物偏好（除其他外）的影响将使用生物体进行研究，这些生物体是研究代谢抑制的优秀模型。该设备将支持的研究因此将具有广泛的生物医学适用性

The equipment requested is a High Resolution Respirometry System for monitoring aerobic activity at low oxygen levels in isolated membranes, mitochondria and cells. For technical reasons until recently, most measurements of cellular and mitochondrial function in vitro are conducted at oxygen levels that substantially exceed in vivo cellular levels. Measurements closer to in vivo oxygen levels are technologically challenging and require very specialized equipment that is now available although from only one manufacturer worldwide. The components are constructed of materials such as titanium to minimize oxygen back flow. The electronic conponents are highly stable for more reliable measurements at low oxygen levels. The system includes a titration/electrode system for montoring mitochondrial membrane potential at low oxygen levels. The equipment will be used to measure a wide range of parameters in isolated mitochondria from estivated (dormant) and active lungfish and snails under low physiological levels of oxygen. This equipment is needed to enable my research program to move forward and verify our hypotheses as to the mechanisms of metabolic depression. The effects of low oxygen levels on mitochondrial oxygen affinity, membrane potential and substrate preferences (among other things) will be studied using organisms that are excellent models for studies of metabolic depression. The research the equipment will support thus will have wide biomedical applicability