A real-time polymerase chain reaction (PCR) system for studies on host-pathogen interactions

用于研究宿主-病原体相互作用的实时聚合酶链反应 (PCR) 系统

• 批准号: 375760-2009
• 负责人: Gottschalk, Marcelo
• 金额: $ 5.13万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2008
• 资助国家: 加拿大
• 起止时间: 2008-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=412224
• 关键词: real; time; polymerase; chain; reaction

The Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP) at the Faculty of Veterinary Medicine (FVM), Univ. de Montréal, is a diversified group of fundamental and applied researchers aimed to increase our knowledge on animal as well as zoonotic diseases. With creation of the FQRNT-granted Swine Infectious Disease Research Centre (CRIP), our research aims were bloated to increase our understanding of the mechanisms involved in the complex interactions of the host with viruses and bacteria of increased significance to the swine industry (including zoonotic agents representing the greatest potential threats to public health) and to apply the knowledge gained to the development of new tools for diagnosis and anti-infectious prophylaxis. Thus, our research involves divergent but inter-connected disciplines, such as molecular biology, host-pathogen interactions, basic immunology and viral and bacterial pathogenesis. In addition, applied research performed by team members focus, among other, on the development of alternative strategies to overcome antibiotic resistance. Many of the research approaches require analysis of cellular responses of specific cell types from diverse hosts (mouse, humans and pigs), for the purpose of studying biological processes experienced by these cells when encountering pathogens. In addition, changes in pathogen behaviour are also experienced, such as expression of new genes involved in antibiotic resistance. Real-time PCR systems, which quantify alterations in mRNA levels of the gene of interest, represent uniquely advantageous tools for achieving these research goals. Indeed, this technology overcomes limitations imposed to our research progress by the lack of appropriated immunological reagents in the field of veterinary diseases, such as specific antibodies. The versatility, high specificity, and accurate quantitative measurements obtained by this technology will greatly facilitate the various research objectives of GREMIP/CRIP-affiliated investigators as well as to achieve a major goal recently fixed by the CRIP: that is to centralize at the FMV a unique porcine immunological tool bank to support our research as well as national and international network.

蒙特利尔大学兽医学院（FVM）的猪疾病研究小组（GREMIP）是一个由基础和应用研究人员组成的多元化小组，旨在增加我们对动物和人畜共患病的知识。随着CRT-granted Swine Infectious Disease Research Centre（CRIP）的成立，我们的研究目标是扩大我们对宿主与病毒和细菌复杂相互作用机制的理解，这对养猪业越来越重要。（包括对公共卫生构成最大潜在威胁的人畜共患病病原体）并将获得的知识应用于诊断和抗感染预防的新工具的开发。因此，我们的研究涉及不同但相互关联的学科，如分子生物学，宿主-病原体相互作用，基础免疫学以及病毒和细菌的发病机制。此外，团队成员进行的应用研究重点是开发克服抗生素耐药性的替代策略。许多研究方法需要分析来自不同宿主（小鼠，人类和猪）的特定细胞类型的细胞反应，以研究这些细胞在遇到病原体时所经历的生物过程。此外，病原体行为也会发生变化，例如与抗生素耐药性相关的新基因的表达。实时PCR系统，它量化的目标基因的mRNA水平的变化，代表了实现这些研究目标的独特的有利工具。事实上，该技术克服了由于在兽医疾病领域缺乏适当的免疫试剂（例如特异性抗体）而对我们的研究进展施加的限制。通过该技术获得的多功能性、高特异性和准确的定量测量将极大地促进GREMIP/CRIP附属研究者的各种研究目标，以及实现CRIP最近确定的主要目标：即在FMV集中建立一个独特的猪免疫学工具库，以支持我们的研究以及国家和国际网络。