Development of a sub-unit vaccine to protect swine against the infections caused by Streptococcus suis

开发亚单位疫苗以保护猪免受猪链球菌引起的感染

• 批准号: 468787-2014
• 负责人: Gottschalk, Marcelo
• 金额: $ 6.69万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Collaborative Research and Development Grants
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=619152
• 关键词: Development; sub; unit; vaccine; protect

Streptococcus suis is one of the most important causes of bacterial disease in post-weaned piglets. Affected animals suffer from meningitis (affected brain), endocarditis (affected heart), arthritis (affected joints) and septicemia (general infection). There are 35 serotypes of S. suis (same bacteria but different antigens). Some of these serotypes (such as serotype 2) can also affect humans (transmission from pigs), causing serious disease. Usually, veterinarians use antibiotics to treat and even protect animals. However, the large use of antibiotic induces the increase of antibiotic-resistance and there are now many S. suis bacterial strains that are resistant. Unfortunately, there are no effective vaccines in the market to protect against this infection. We have identified a protein called "Sao" that protects pigs against a bacterial strain of S. suis serotype 2. It is not known if it protects against other strains and other serotypes that cause disease in pigs. Production of Sao protein should also be improved to obtain acceptable yields to have enough material to be tested in a large vaccine trial. In addition, this protein should be mixed with a very good pharmacological agent that increase or aid the immune response of animals (called "adjuvant"). A good adjuvant for Sao protein that is allowed to be commercially used in pigs needs to be found.The general objective of this project is to obtain a reliable Sao-adjuvant prototype vaccine that will protect pigs against most serotypes of S. suis. In addition, and to complete the vaccine, other proteins already identified by our laboratory will be tested and incorporated into the vaccine. At the end of the project we will have a vaccine prototype composed by Sao combined with other protein(s) with an appropriate adjuvant.

猪链球菌是引起断奶仔猪细菌性疾病的重要病原之一。受影响的动物患有脑膜炎（受影响的大脑），心内膜炎（受影响的心脏），关节炎（受影响的关节）和败血症（全身感染）。有35个血清型。猪（相同的细菌但不同的抗原）。其中一些血清型（如血清型2）也可以影响人类（从猪传播），导致严重的疾病。通常，兽医使用抗生素来治疗甚至保护动物。然而，抗生素的大量使用导致了耐药性的增加，目前已经出现了许多沙门氏菌。具有耐药性的猪细菌菌株。不幸的是，市场上没有有效的疫苗来预防这种感染。我们已经发现了一种名为“Sao”的蛋白质，它可以保护猪免受沙门氏菌菌株的侵害。猪血清2型目前尚不清楚它是否能预防引起猪疾病的其他菌株和其他血清型。还应改进Sao蛋白的生产，以获得可接受的产量，从而在大型疫苗试验中有足够的材料进行测试。此外，这种蛋白质应该与一种非常好的药理学试剂混合，以增加或帮助动物的免疫反应（称为“佐剂”）。本项目的总体目标是获得一种可靠的Sao佐剂原型疫苗，该疫苗将保护猪抵抗大多数血清型的S。猪。此外，为了完成疫苗，我们实验室已经鉴定的其他蛋白质将被测试并纳入疫苗中。在项目结束时，我们将有一个疫苗原型，由Sao与其他蛋白质结合适当的佐剂组成。