Responses of dorsal root ganglion cells to increased and decreased neuromuscular activity

背根神经节细胞对神经肌肉活动增加和减少的反应

• 批准号: 6644-2007
• 负责人: Gardiner, Phillip
• 金额: $ 3.89万
• 依托单位: University of Manitoba
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=420283
• 关键词: Responses; dorsal; root; ganglion; cells

Very little is known as to the effects of increased and decreased physical activity on the properties of sensory nerves. The proposed research program will examine the large sensory cells innervating mechanoreceptors in the hindlimbs of rats exposed to 1 month of increased (treadmill training and voluntary wheel exercise) or decreased (unilateral hindlimb paralysis and simulated weightlessness via hindlimb suspension) daily neuromuscular activity. Properties to be measured in dorsal root ganglion cells include their electrophysiological properties and ion current densities, the expression of genes coding for neurotrophic factors and ion channel subunits, their metabolic signaling responses to acute electrical stimulation, and their synaptic efficacy with alpha-motoneurones in spinal cord. Techniques to be used range from electrophysiological to immunohistochemical, and on to procedures used to detect changes in gene expression. The information emanating from these experiments will give us some direction in examining physical activity effects on other types of sensory cells, such as pain afferents, and in more complex systems, such as brain and spinal cord.

人们对增加和减少体力活动对感觉神经特性的影响知之甚少。 拟议的研究计划将检查大鼠后肢中支配机械感受器的大感觉细胞，这些大鼠暴露于1个月的增加（跑步机训练和自愿轮运动）或减少（单侧后肢瘫痪和通过后肢悬吊模拟失重）的日常神经肌肉活动。 在背根神经节细胞中待测量的特性包括它们的电生理特性和离子电流密度、编码神经营养因子和离子通道亚基的基因的表达、它们对急性电刺激的代谢信号传导响应以及它们与脊髓中的α-运动神经元的突触功效。 所使用的技术范围从电生理学到免疫组织化学，以及用于检测基因表达变化的程序。 从这些实验中产生的信息将为我们研究身体活动对其他类型感觉细胞（如疼痛传入）以及更复杂系统（如大脑和脊髓）的影响提供一些指导。