Clarification of dorsal root evoked synaptic responses in lamina I neurons

阐明 I 层神经元背根诱发的突触反应

• 批准号: 21600005
• 负责人: YOSHIMURA Megumu
• 金额: $ 3万
• 依托单位: Kumamoto Health Science University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21600005/
• 关键词: slow EPSP; サブスタンスP; 後角第I層; 投射ニューロン; 脊髄第I層; 緩徐シナプス応答; C線維; ペプチド; 痛覚伝達; 脊髄後角; 痛覚過敏; 脊髄スライス; slow EPSC; 痛覚; 脊髄スライス標本; 無髄のC線維

Intracellular recordings were made from lamina I neurons which were identified by antidromic action potentials evoked by focal stimulation near the central canal. Stimulation of the dorsal root elicited a fast EPSP in the majority of neurons and repetitive stimulation evoked a slow EPSP in 20% of lamina I neurons. The latter neurons were depolarized by application of substance P and antagonized by a NK1 receptor antagonist, suggesting that the lamina I neurons receive substance P containing C afferent fibers which could modulate the nociceptive transmission in the lamina I.

细胞内记录是由椎板I神经元制成的，这些神经元是通过中央管附近的局灶性刺激引起的抗肿瘤作用电位鉴定出来的。对背根的刺激在大多数神经元中引起了快速EPSP，重复刺激引起了20％的lamina I神经元的缓慢EPSP。通过施用PESTS P将后一种神经元去极化，并通过NK1受体拮抗剂拮抗，这表明薄片I神经元接收到含有C传感纤维的物质，这些物质可以调节层中的伤害性传播。

项目成果

TRPA1-expressing primary afferents synapse with morphologically identifird subtypes f substantia gelatinosa neurons in the adult rat spinal cord.

成年大鼠脊髓中胶质质神经元中表达 TRPA1 的初级传入突触具有形态学上相同的亚型。

• 发表时间: 2010
• 期刊: European Journal of Neuroscience. (In press)
• 作者: Uta D;Furue H;Pickering AE;Rashid MDH;Mizuguchi-Takase H;Katafuchi T;Yoshimura M.
• 通讯作者: Yoshimura M.

Functional role of the TRPV1 receptors expressed at the terminal of prmary C afferent in the spinal dorsal hor

脊髓背角初级 C 传入神经末端表达的 TRPV1 受体的功能作用

• 发表时间: 2010
• 作者: Yoshimura M;Xie D;Furue H;Hachisuka J;Uta D
• 通讯作者: Uta D

Responsiveness of C Neurons in Rat Dorsal Root Ganglion to 5-Hydroxytryptamine-Induced Pruritic Stimuli In Vivo

• DOI: 10.1152/jn.00938.2009
• 发表时间: 2010-07-01
• 期刊: JOURNAL OF NEUROPHYSIOLOGY
• 影响因子: 2.5
• 作者: Hachisuka, Junichi;Furue, Hidemasa;Yoshimura, Megumu
• 通讯作者: Yoshimura, Megumu

標準生理学、体性感覚

标准生理学、体感

• 发表时间: 2009
• 作者: H. Mizoguchi;C. Watanabe;S. Osada;M. Yoshioka;Y. Aoki;S. Natsui;A. Yonezawa;S. Kanno;M. Ishikawa;T. Sakurada;S. Sakurada;Watanabe S.;吉村惠(分担)
• 通讯作者: 吉村惠(分担)

Modulation of nociceptive synaptic transmission in the adult rat spinal dorsal horn via activation of TPA1 and TRPV1

通过激活 TPA1 和 TRPV1 调节成年大鼠脊髓背角的伤害性突触传递

• 发表时间: 2009
• 作者: Uta D;Furue H;Yoshimura M
• 通讯作者: Yoshimura M