Structure and function of acyl carrier protein

酰基载体蛋白的结构和功能

• 批准号: 155331-2008
• 负责人: Byers, David
• 金额: $ 3.34万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2009
• 资助国家: 加拿大
• 起止时间: 2009-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=422519
• 关键词: Structure; function; acyl; carrier; protein

My research is trying to discover how acyl carrier protein (ACP), a key central protein in bacterial fatty acid synthesis, can recognize and discriminate among its multiple protein partners. These partners include over three dozen enzymes involved in synthesis of membrane lipids, toxins (eg. endotoxin), and other essential components of bacterial growth and pathogenesis. ACP must fold to enclose attached fatty acids as it carries them through the watery environment of the cell, but must then unfold to release them upon interaction with ACP-dependent enzymes. We believe that ACP is a special flexible and dynamic protein, with specific amino acids important for enzyme binding and triggering the unfolding and release of the acyl chain.

我的研究试图发现酰基载体蛋白(ACP)，一种细菌脂肪酸合成的关键中心蛋白质，如何在其多个蛋白质伙伴中识别和区分。这些伙伴包括30多种参与膜脂、毒素合成的酶(例如。内毒素)，以及细菌生长和致病的其他基本成分。ACP必须折叠以封闭附着的脂肪酸，因为它携带它们穿过细胞的水环境，但随后必须展开以在与ACP依赖的酶相互作用时释放它们。我们认为，ACP是一种特殊的柔性和动态蛋白质，具有特定的氨基酸，对酶结合和触发酰链的展开和释放非常重要。