Spectrofluorometer for protein structure and function analysis

用于蛋白质结构和功能分析的分光荧光计

• 批准号: 359447-2008
• 负责人: Byers, David
• 金额: $ 5.82万
• 依托单位: Dalhousie University
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2007
• 资助国家: 加拿大
• 起止时间: 2007-01-01 至 2008-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=364003
• 关键词: Spectrofluorometer; protein; structure; function; analysis

Proteins are the most functionally diverse biomolecules, and our collective research is trying to understand how they work.  The proteins we study include a key protein involved in bacterial growth and pathogenesis, enzymes that are essential for cellular metabolism, membrane receptors, and collagen, the most abundant animal protein in the world.  Our work will lead not only to greater knowledge of important biological processes, but also to development of new products and therapies for many diseases such as cancer and infection.As proteins are much too small to be seen, our research relies on special probes to report what is going on at the atomic to molecular level. One of the most useful tools for protein research is fluorescence, which can provide accurate and sensitive information about molecular environment, interactions, and dynamic events. The requested spectrofluorometer will allow us to investigate these properties for proteins with natural or attached fluorescent probes, using state-of-the-art accessories that can provide information about the dynamic events involved in protein structural changes and interactions.

蛋白质是功能最多样的生物分子，我们的集体研究正在试图了解它们是如何工作的。我们研究的蛋白质包括一种参与细菌生长和发病的关键蛋白质、细胞新陈代谢所必需的酶、膜受体以及世界上含量最丰富的动物蛋白质--胶原蛋白。我们的工作不仅将使我们更好地了解重要的生物过程，还将为癌症和感染等许多疾病开发新产品和治疗方法。由于蛋白质太小而看不见，我们的研究依赖于特殊的探针来报告原子到分子水平上正在发生的事情。蛋白质研究最有用的工具之一是荧光，它可以提供关于分子环境、相互作用和动态事件的准确和敏感的信息。所要求的荧光分光光度计将使我们能够使用最先进的配件来研究带有天然或附加荧光探针的蛋白质的这些性质，这些配件可以提供涉及蛋白质结构变化和相互作用的动态事件的信息。