A three-tiered strategy to prevent destabilisation of the induced pluripotent stem cell genome by L1 retrotransposition

防止 L1 逆转录转座导致诱导多能干细胞基因组不稳定的三层策略

• 批准号: nhmrc : 1067983
• 负责人: Dr Kyle Upton
• 金额: $ 47.26万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Project Grants
• 财政年份: 2014
• 资助国家: 澳大利亚
• 起止时间: 2014-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/three-tiered-strategy-l1-retrotransposition/455327
• 关键词: three; tiered; strategy; prevent; destabilisation

Retrotransposons are mobile genes that copy-and-paste themselves in our genome. Previously thought to represent “junk DNA”, retrotransposons are increasingly recognised to play major roles in biology. In recent publications in Cell and Nature, we found that retrotransposons move in mature human cells. In the current study, we will determine whether the same activity occurs when these cells are reprogrammed to become stem cells, possibly impacting stem cell based therapies.

反转录转座子是一种可移动的基因，可以在我们的基因组中复制和粘贴自己。反转录转座子以前被认为是“垃圾DNA”，现在越来越多地被认为在生物学中发挥着重要作用。在最近发表在《细胞》和《自然》杂志上的文章中，我们发现反转录转座子在成熟的人类细胞中运动。在目前的研究中，我们将确定当这些细胞被重新编程成为干细胞时，是否会发生同样的活动，这可能会影响基于干细胞的治疗。